• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一对部分非互补的肽核酸对双链DNA中错配位点的识别

Recognition of mismatched sites in double-stranded DNA by a pair of partially noncomplementary peptide nucleic acids.

作者信息

Shibata Masanari, Shoji Osami, Aiba Yuichiro

机构信息

Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8602, Japan.

出版信息

Chem Lett. 2024 Dec 10;53(12):upae234. doi: 10.1093/chemle/upae234. eCollection 2024 Dec.

DOI:10.1093/chemle/upae234
PMID:39677325
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11640769/
Abstract

We have successfully achieved efficient recognition of mismatched sites in double-stranded DNA through the formation of an invasion complex by using partially noncomplementary peptide nucleic acids (PNAs). Owing to mismatches between 2 PNAs used for invasion, the undesired PNA/PNA duplex, which inhibits invasion complex formation, was destabilized. This approach overcame an inherent challenge in PNA invasion, in particular, undesired PNA/PNA duplex formation resulting from PNA complementarity, thereby enhancing overall invasion efficiency.

摘要

我们通过使用部分非互补肽核酸（PNA）形成入侵复合物，成功实现了对双链DNA中错配位点的高效识别。由于用于入侵的两个PNA之间存在错配，抑制入侵复合物形成的不期望的PNA/PNA双链体变得不稳定。这种方法克服了PNA入侵中固有的挑战，特别是由PNA互补性导致的不期望的PNA/PNA双链体形成，从而提高了整体入侵效率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/e2a57db5a439/upae234f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/f2055449250f/upae234_ga.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/68a8e267628b/upae234f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/14d8da921f2f/upae234f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/5e16929c578d/upae234f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/e2a57db5a439/upae234f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/f2055449250f/upae234_ga.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/68a8e267628b/upae234f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/14d8da921f2f/upae234f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/5e16929c578d/upae234f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ed5/11640769/e2a57db5a439/upae234f4.jpg

相似文献

1
Recognition of mismatched sites in double-stranded DNA by a pair of partially noncomplementary peptide nucleic acids.一对部分非互补的肽核酸对双链DNA中错配位点的识别
Chem Lett. 2024 Dec 10;53(12):upae234. doi: 10.1093/chemle/upae234. eCollection 2024 Dec.
2
Peptide Nucleic Acid Conjugated with Ruthenium-Complex Stabilizing Double-Duplex Invasion Complex Even under Physiological Conditions.肽核酸与钌配合物偶联稳定双螺旋入侵复合物，即使在生理条件下也是如此。
Chembiochem. 2018 Aug 6;19(15):1601-1604. doi: 10.1002/cbic.201800256. Epub 2018 Jun 27.
3
Extending recognition by peptide nucleic acids (PNAs): binding to duplex DNA and inhibition of transcription by tail-clamp PNA-peptide conjugates.肽核酸（PNA）扩展识别作用：与双链DNA结合以及尾夹PNA-肽缀合物对转录的抑制作用
Biochemistry. 2003 Dec 2;42(47):13996-4003. doi: 10.1021/bi035194k.
4
An experimental study of mechanism and specificity of peptide nucleic acid (PNA) binding to duplex DNA.肽核酸（PNA）与双链DNA结合的机制及特异性的实验研究。
J Mol Biol. 1999 Mar 12;286(5):1337-45. doi: 10.1006/jmbi.1998.2578.
5
Incorporation of thio-pseudoisocytosine into triplex-forming peptide nucleic acids for enhanced recognition of RNA duplexes.将硫代假异胞嘧啶掺入三链形成肽核酸中以增强对RNA双链体的识别。
Nucleic Acids Res. 2014 Apr;42(6):4008-18. doi: 10.1093/nar/gkt1367. Epub 2014 Jan 13.
6
Combined triplex/duplex invasion of double-stranded DNA by "tail-clamp" peptide nucleic acid.“尾夹”肽核酸对双链DNA的三联体/双链体联合侵入
Biochemistry. 2003 Dec 2;42(47):13987-95. doi: 10.1021/bi0351918.
7
A simple fluorescent assay for the detection of peptide nucleic acid-directed double strand duplex invasion.一种用于检测肽核酸导向双链体入侵的简单荧光检测法。
Biopolymers. 2022 Jan;113(1):e23475. doi: 10.1002/bip.23475. Epub 2021 Sep 16.
8
Strand invasion by mixed base PNAs and a PNA-peptide chimera.混合碱基肽核酸及肽核酸-肽嵌合体的链侵入
Nucleic Acids Res. 2000 Sep 1;28(17):3332-8. doi: 10.1093/nar/28.17.3332.
9
Chiral introduction of positive charges to PNA for double-duplex invasion to versatile sequences.通过对手性正电荷引入肽核酸（PNA）实现对多种序列的双双链入侵。
Nucleic Acids Res. 2008 Mar;36(5):1464-71. doi: 10.1093/nar/gkm1154. Epub 2008 Jan 18.
10
Mismatch recognition in PNA double-duplex invasion.肽核酸双双链侵入中的错配识别
Nucleic Acids Symp Ser (Oxf). 2008(52):125-6. doi: 10.1093/nass/nrn064.

本文引用的文献

1
Recent Advancements in Development and Therapeutic Applications of Genome-Targeting Triplex-Forming Oligonucleotides and Peptide Nucleic Acids.基因组靶向三链形成寡核苷酸和肽核酸的开发及治疗应用的最新进展
Pharmaceutics. 2023 Oct 23;15(10):2515. doi: 10.3390/pharmaceutics15102515.
2
Metal-mediated DNA strand displacement and molecular device operations based on base-pair switching of 5-hydroxyuracil nucleobases.基于 5-羟尿嘧啶碱基对切换的金属介导 DNA 链置换和分子器件操作。
Nat Commun. 2023 Aug 24;14(1):4759. doi: 10.1038/s41467-023-40353-3.
3
Triplex-Forming Peptide Nucleic Acid Controls Dynamic Conformations of RNA Bulges.
三聚体形成肽核酸控制 RNA 突环的动态构象。
J Am Chem Soc. 2023 May 17;145(19):10497-10504. doi: 10.1021/jacs.2c12488. Epub 2023 May 8.
4
Kinetic analysis of highly effective triplex formation between a small molecule-peptide nucleic acid conjugate probe and the influenza A virus RNA promoter region at neutral pH.在中性pH条件下，小分子-肽核酸共轭探针与甲型流感病毒RNA启动子区域之间高效三链体形成的动力学分析。
Org Biomol Chem. 2023 Apr 26;21(16):3402-3410. doi: 10.1039/d3ob00262d.
5
Unique Crosslinking Properties of Psoralen-Conjugated Oligonucleotides Developed by Novel Psoralen N-Hydroxysuccinimide Esters.新型补骨脂素N-羟基琥珀酰亚胺酯开发的补骨脂素共轭寡核苷酸的独特交联特性。
Chembiochem. 2023 Aug 1;24(15):e202200789. doi: 10.1002/cbic.202200789. Epub 2023 May 23.
6
Pseudo-Complementary G:C Base Pair for Mixed Sequence dsDNA Invasion and Its Applications in Diagnostics (SARS-CoV-2 Detection).用于混合序列双链DNA侵入的伪互补G:C碱基对及其在诊断中的应用（严重急性呼吸综合征冠状病毒2检测）
JACS Au. 2023 Feb 1;3(2):449-458. doi: 10.1021/jacsau.2c00588. eCollection 2023 Feb 27.
7
In vivo correction of cystic fibrosis mediated by PNA nanoparticles.肽核酸纳米颗粒介导的囊性纤维化体内校正。
Sci Adv. 2022 Oct 7;8(40):eabo0522. doi: 10.1126/sciadv.abo0522. Epub 2022 Oct 5.
8
Selective Chemical Modification to the Higher-Order Structures of Nucleic Acids.选择性化学修饰核酸的高级结构。
Chem Rec. 2023 Feb;23(2):e202200194. doi: 10.1002/tcr.202200194. Epub 2022 Sep 16.
9
NMR determination of the 2:1 binding complex of naphthyridine carbamate dimer (NCD) and CGG/CGG triad in double-stranded DNA.NMR 测定萘啶基氨基甲酸酯二聚体（NCD）与双链 DNA 中 CGG/CGG 三核苷酸的 2:1 结合复合物。
Nucleic Acids Res. 2022 Sep 23;50(17):9621-9631. doi: 10.1093/nar/gkac740.
10
Triple-Helical Binding of Peptide Nucleic Acid Inhibits Maturation of Endogenous MicroRNA-197.三螺旋结合肽核酸抑制内源性 microRNA-197 的成熟。
ACS Chem Biol. 2021 Jul 16;16(7):1147-1151. doi: 10.1021/acschembio.1c00133. Epub 2021 Jun 11.