兔肿瘤坏死因子的纯化
Purification of rabbit tumor necrosis factor.
作者信息
Abe S, Gatanaga T, Yamazaki M, Soma G, Mizuno D
出版信息
FEBS Lett. 1985 Jan 28;180(2):203-6. doi: 10.1016/0014-5793(85)81071-1.
Rabbit tumor necrosis factor (TNF) was purified and shown by SDS-PAGE to be a single protein of 18 kDa. TNF in 355 ml rabbit serum was precipitated with ammonium sulfate, and purified by repeated DEAE-Sephadex and Sephacryl S-200 chromatographies, and the final fractionation on Blue-Sepharose 6B. By this procedure its yield was 22% and its specific activity was 2.4 X 10(7) U/mg protein. The sequence of the N-terminal 20 amino acids was determined.
兔肿瘤坏死因子（TNF）经纯化后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）显示为一种分子量为18 kDa的单一蛋白质。用硫酸铵沉淀355 ml兔血清中的TNF，然后通过重复的二乙氨基乙基-葡聚糖凝胶（DEAE-Sephadex）和Sephacryl S-200柱层析以及最终在Blue-Sepharose 6B上的分级分离进行纯化。通过该方法，其产率为22%，比活性为2.4×10⁷ U/mg蛋白质。测定了其N端20个氨基酸的序列。
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