Yoneda K, Osaki T, Yamamoto T, Ueta E
Department of Oral Surgery, Kochi Medical School, Japan.
Clin Exp Immunol. 1993 Aug;93(2):229-36. doi: 10.1111/j.1365-2249.1993.tb07971.x.
Roles of monocytes and cytokines were investigated on LAK induction from T and NK cells. Monocytes augmented more T-LAK induction than did NK-LAK. Expression of IL-1 beta, TNF-alpha and interferon-gamma (IFN-gamma)-mRNA and their cytokine production were superior in NK cells compared with T cells in parallel with their LAK activities. An increase of TNF-alpha, IL-1 beta and IFN-gamma production was induced by co-culturing NK or T cells with autologous monocytes. The augmentation of T cell cytokine production and T-LAK activity by monocytes was more prominent than that of NK cells. TNF-alpha and IL-1 beta were generated 24 h after IL-2 stimulation, and these cytokines were able to almost substitute for monocytes in LAK induction. Conversely, LAK induction was almost completely suppressed by both anti-IL-1 beta and anti-TNF-alpha antibodies, if they were added within 24 h after the start of the LAK induction. IFN-gamma, which was produced at a later stage, scarcely affected LAK induction in spite of the cooperation with TNF-alpha. The results obtained indicate conclusively that the superiority of NK-LAK depends on their superior productivity of both IL-1 beta and TNF-alpha, and that the up-regulation of LAK induction by monocytes is largely due to the enhanced generation of both cytokines.
研究了单核细胞和细胞因子在从T细胞和NK细胞诱导LAK中的作用。单核细胞对T-LAK诱导的增强作用比NK-LAK更强。与T细胞相比，NK细胞中IL-1β、TNF-α和干扰素-γ（IFN-γ）-mRNA的表达及其细胞因子产生与其LAK活性平行，更为优越。通过将NK或T细胞与自体单核细胞共培养，可诱导TNF-α、IL-1β和IFN-γ产生增加。单核细胞对T细胞细胞因子产生和T-LAK活性的增强作用比NK细胞更为显著。TNF-α和IL-1β在IL-2刺激后24小时产生，这些细胞因子在LAK诱导中几乎能够替代单核细胞。相反，如果在LAK诱导开始后24小时内加入抗IL-1β和抗TNF-α抗体，则LAK诱导几乎完全被抑制。后期产生的IFN-γ尽管与TNF-α协同作用，但几乎不影响LAK诱导。所得结果确凿地表明，NK-LAK的优越性取决于其IL-1β和TNF-α的更高产生能力，并且单核细胞对LAK诱导的上调在很大程度上归因于这两种细胞因子产生的增强。
