Claybaugh J R, Sato A K
Fed Proc. 1985 Jan;44(1 Pt 1):62-5.
Vasopressin (VP) activity has been measured in urine for assessments of long-term changes in hormone release. Most laboratories employ radioimmunoassays (RIAs) and extraction of urine for research. It has been assumed that the specificity of the antiserums used and the extraction procedures would be adequate to safeguard against non-VP substances that would interfere with the RIA. New evidence is presented that this is probably not the case. High-performance liquid chromatography of unextracted urine or CG-50 extracts of urine clearly separates two major immunologically active peaks. One corresponds to native VP and the other migrates with the front. Different antiserums recognize the non-VP peak variably, but the VP peak the same. These activities were not separated by Sephadex G-25 chromatography. In addition to variability arising in the measurement of VP, the VP excreted may be influenced by variable renal clearance of the hormone. There is ample evidence to assume that not all plasma VP is filterable and that the proportions of bound and unbound VP vary. Furthermore, there is tubular metabolism and secretion of VP. The variability of these functions is unknown.
已对尿液中的血管加压素（VP）活性进行测量，以评估激素释放的长期变化。大多数实验室采用放射免疫分析法（RIA）并对尿液进行提取用于研究。人们一直认为所使用抗血清的特异性和提取程序足以防止干扰RIA的非VP物质。本文提出的新证据表明情况可能并非如此。对未提取的尿液或尿液的CG - 50提取物进行高效液相色谱分析可清晰分离出两个主要的免疫活性峰。一个对应天然VP，另一个与前沿一起迁移。不同的抗血清对非VP峰的识别程度不同，但对VP峰的识别相同。这些活性物质不能通过Sephadex G - 25色谱法分离。除了VP测量中出现的变异性外，排泄的VP可能受该激素肾脏清除率变化的影响。有充分证据表明并非所有血浆VP都可滤过，且结合型和未结合型VP的比例各不相同。此外，存在VP的肾小管代谢和分泌。这些功能的变异性尚不清楚。
