Ramdhony Kamlesh, Puchooa Daneshwar, Faraj Turki Kh, Alrefaei Abdulwahed Fahad, Li JunFu, Jeewon Rajesh
Department of Agricultural and Food Science, Faculty of Agriculture, University of Mauritius, Réduit, Mauritius.
Department of Soil Science, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
PeerJ. 2024 Dec 12;12:e18325. doi: 10.7717/peerj.18325. eCollection 2024.
Bioprospecting robust and oleaginous strain is crucial for the commercialization of microalgae-based biodiesel. In this study, a microalgal strain SCH18 was isolated from a solar saltern located in Mauritius. This isolate was identified as based on a polyphasic approach that combined molecular, physiological, and morphological analyses. Furthermore, the effect of different salinities on the biochemical composition and fatty acid profile of this microalga was investigated to explore its potential in producing biodiesel. Results from the growth studies showed that salinity of 1.0 M NaCl was optimal for achieving a high growth rate. Under this salt concentration, the growth rate and the doubling time were calculated as 0.39 ± 0.003 day and 1.79 ± 0.01 days, respectively. In terms of biochemical composition, a substantial amount of carbohydrate (42.02 ± 5.20%), moderate amount of protein (30.35 ± 0.18%) and a low lipid content (17.81 ± 2.4%) were obtained under optimal NaCl concentration. The fatty acid analysis indicated the presence of palmitic acid, stearic acid, palmitoleic acid, oleic acid, linoleic acid, gamma, and alpha-linolenic acids, which are suitable for biodiesel synthesis. The predicted biodiesel properties were in accordance with the standard of ASTM 6751, indicating that the microalgal isolate . SCH18 is a potential candidate for use in biodiesel production.
筛选出健壮且富含油脂的微藻菌株对于基于微藻的生物柴油商业化至关重要。在本研究中，从毛里求斯的一个太阳能盐场分离出了一株微藻菌株SCH18。基于分子、生理和形态学分析相结合的多相方法，该分离株被鉴定出来。此外，研究了不同盐度对这种微藻生化组成和脂肪酸谱的影响，以探索其在生物柴油生产中的潜力。生长研究结果表明，1.0 M NaCl的盐度最有利于实现高生长速率。在这种盐浓度下，生长速率和倍增时间分别计算为0.39±0.003天和1.79±0.01天。在生化组成方面，在最佳NaCl浓度下，获得了大量的碳水化合物（42.02±5.20%）、适量的蛋白质（30.35±0.18%）和较低的脂质含量（17.81±2.4%）。脂肪酸分析表明存在棕榈酸、硬脂酸、棕榈油酸、油酸、亚油酸、γ-亚麻酸和α-亚麻酸，这些都适合用于生物柴油合成。预测的生物柴油特性符合ASTM 6751标准，表明微藻分离株SCH18是生物柴油生产的潜在候选菌株。
