Jiangsu Provincial Key Laboratory of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China.
Sci Rep. 2017 Aug 14;7(1):8118. doi: 10.1038/s41598-017-07540-x.
The biofuels are receiving considerable attention as a substitute for petro diesel. For microalgae, the cell density or biomass and lipid contents are key components for biodiesel production. This study was conducted to develop favorable culture conditions for Dunaliella salina to maximize its biomass and lipid accumulation. The effect of salinity (0.5 to 2.5 M NaCl) on the cell population, biochemical composition, and lipid output of Dunaliella salina was examined under a controlled environment for 21 days. Maximum growth (6.57 × 10 to 7.17 × 10cells mL) potentials were observed at 1.5 to 2 M NaCl. The photosynthetic pigments and carbohydrates also showed trends similar to growth. The maximum carotenoid level (5.16 mg L) was recorded at 2 M NaCl. Almost all physicochemical parameters increased with increases in salinity, biomass (1231.66 ± 1.26 mg L) and lipid content (248.33 mg L), as recorded at 2 M NaCl. Based on fluorescence intensity, the highest values (11.84 × 10cells/ml) of neutral lipids and total lipids (22.28%) were recorded at optimum salinity levels. The present study suggests that a high biomass and lipid accumulation of Dunaliella salina SA 134 could be obtained at the 2 M NaCl level.
生物燃料作为石油柴油的替代品受到了相当大的关注。对于微藻而言，细胞密度或生物量和脂质含量是生物柴油生产的关键组成部分。本研究旨在开发适合杜氏盐藻的有利培养条件，以最大限度地提高其生物量和脂质积累。在受控环境下，研究了盐度（0.5 至 2.5 M NaCl）对杜氏盐藻细胞种群、生物化学组成和脂质产量的影响，持续 21 天。在 1.5 至 2 M NaCl 下观察到最大生长（6.57×10 至 7.17×10 个细胞/mL）潜力。光合色素和碳水化合物也表现出与生长相似的趋势。最大类胡萝卜素水平（5.16 mg/L）记录在 2 M NaCl 下。随着盐度的增加，几乎所有理化参数均增加，生物量（1231.66±1.26 mg/L）和脂质含量（248.33 mg/L）最高，记录在 2 M NaCl 下。根据荧光强度，中性脂质和总脂质（22.28%）的最高值（11.84×10 个细胞/ml）记录在最佳盐度水平下。本研究表明，在 2 M NaCl 水平下，可以获得杜氏盐藻 SA 134 的高生物量和脂质积累。
