Takeuchi Shiori, Imai Shosei, Terai Takuya, Campbell Robert E
Department of Chemistry, Graduate School of Science, The University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan
CERVO Brain Research Center and Department of Biochemistry, Microbiology, and Bioinformatics, Université Laval, Québec Québec G1V 0A6 Canada.
RSC Chem Biol. 2024 Dec 3;6(2):170-174. doi: 10.1039/d4cb00256c. eCollection 2025 Feb 5.
A chemigenetic indicator with an affinity suitable for imaging of intracellular sodium ions (Na) in mammalian cells was developed. The indicator, based on a chimera of green fluorescent protein (GFP) and HaloTag labeled with a synthetic crown ether chelator, was produced by a combination of rational design and directed evolution. In mammalian cells the indicator exhibited an approximately 100% increase in excitation ratio when the cells were treated with 20 mM Na and an ionophore.
开发了一种化学遗传指示剂，其亲和力适合对哺乳动物细胞内的钠离子（Na）进行成像。该指示剂基于绿色荧光蛋白（GFP）和标记有合成冠醚螯合剂的HaloTag的嵌合体，通过合理设计和定向进化相结合的方式产生。在哺乳动物细胞中，当用20 mM Na和离子载体处理细胞时，该指示剂的激发比增加了约100%。
