Palpperumal Selvam, Sankaralingam Subbiah, Balachandran Chellapandian, Mahendran Shunmugiah, Venkatesh Sakthivel, Alharbi Naiyf S, Thiruvengadam Muthu, Duraipandiyan Veeramuthu, Baskar Kathirvelu
PG and Research Department of Microbiology, V. H. N. Senthikumara Nadar College (Autonomous), Virudhunagar, Tamil Nadu India.
PG and Research Department of Botany, Saraswathi Narayanan College, Madurai, Tamil Nadu India.
Indian J Microbiol. 2024 Dec;64(4):1805-1825. doi: 10.1007/s12088-024-01237-2. Epub 2024 Apr 21.
The emergence of various diseases such as cardiovascular, aging and cancer are due to oxidative stress as well as the shortage of enough antioxidant materials. Our study was intended to analyze the anticancer, antimicrobial, wound healing as well as hepatoprotective activities of purified sulfated polysaccharides derived from The sulfated polysaccharide was subjected to partial purification using DEAE cellulose. The sulfated fraction was identified by HPLC, FTIR, H-NMR and GC-MS. The results showed that the presence of fucopyranose in sulfated polysaccharide was attached to the - groups confirmed by H-NMR analysis. Further was carried out for the antioxidant profiling of sulfated polysaccharide through various standard assays. Bioactive principles of sulfated polysaccharides in medicinal fields were confirmed by anticancer activity on MDA-MB-231 breast cancer cells and wound healing activity in four groups of albino rats. The hepatoprotective activity of sulfated fraction was determined based on their biochemical parameters. Emulsification activity of sulfated polysaccharide was also evaluated. Also, it has antimicrobial properties. Our report has pointed out that the supplementation of sulfated polysaccharides would prevent the formation of cancer and oxidative damage of biomolecules.
心血管疾病、衰老和癌症等各种疾病的出现是由于氧化应激以及足够的抗氧化物质短缺所致。我们的研究旨在分析从[未提及具体来源]中提取的纯化硫酸化多糖的抗癌、抗菌、伤口愈合以及肝脏保护活性。使用DEAE纤维素对硫酸化多糖进行部分纯化。通过HPLC、FTIR、H-NMR和GC-MS对硫酸化部分进行鉴定。结果表明，通过H-NMR分析证实硫酸化多糖中存在岩藻吡喃糖并与-基团相连。进一步通过各种标准测定法对硫酸化多糖的抗氧化特性进行了分析。硫酸化多糖在医学领域的生物活性原理通过对MDA-MB-231乳腺癌细胞的抗癌活性和四组白化大鼠的伤口愈合活性得到证实。基于其生化参数测定了硫酸化部分的肝脏保护活性。还评估了硫酸化多糖的乳化活性。此外，它具有抗菌特性。我们的报告指出，补充硫酸化多糖可预防癌症的形成和生物分子的氧化损伤。
