Ommen Pernille, Zobek Natalia, Meyer Rikke Louise
Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
Interdisciplinary Nanoscience Center, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark; Department of Bioscience, Aarhus University, Ny Munkegade 114, 8000 Aarhus C, Denmark.
J Microbiol Methods. 2017 Oct;141:87-89. doi: 10.1016/j.mimet.2017.08.003. Epub 2017 Aug 10.
Crystal violet staining is commonly used for quantification of biofilm formation, although it is highly toxic. Here we test safranin as a non-toxic replacement. Safranin staining provided similar results as crystal violet, but with higher reproducibility. We therefore recommend safranin staining for biofilm biomass quantification.
结晶紫染色常用于生物膜形成的定量分析，尽管它具有高毒性。在此，我们测试了番红作为一种无毒替代品。番红染色提供了与结晶紫相似的结果，但具有更高的重现性。因此，我们推荐使用番红染色进行生物膜生物量的定量分析。
