Bhowmik Banasree, Afrin Sadia, Jui Afsana Habib, Bhuiyan Riyadh Hossen, Rashid Md Mamunur, Miah Md Abdus Satter, Bhuiyan Mohammad Nazrul Islam
Principal Scientific Officer (PSO), Industrial Microbiology Laboratory, Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhaka, 1205, Bangladesh.
BCSIR Dhaka Laboratories, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dr. Qudrat-i-Khuda Road, Dhaka, 1205, Bangladesh.
Mol Biol Rep. 2024 Dec 16;52(1):51. doi: 10.1007/s11033-024-10173-9.
Exopolysaccharides (EPS) derived from microbial sources hold great promise for various industrial applications due to their biodegradability and diverse biological activities.
In this study, EPS was isolated and characterized from Bacillus amyloliquefaciens strain BDIFST240014, a Gram-positive bacterium known for its robust EPS production. Taxonomic classification was confirmed through 16 S rRNA gene sequencing. We optimized the growth conditions for EPS production, yielding a maximum of approximately 74.81 mg/L after 48 h of incubation at 37 °C. UV-vis and FTIR spectroscopy were used to characterize the EPS, revealing key functional groups indicative of its polysaccharide nature. The EPS contained total carbohydrate and protein contents of 841.234 µg/5 mg and 630.54 µg/5 mg, respectively, along with a potent water holding capacity (WHC) of 103.31% and oil holding capacity (OHC) of 106.78%, underscoring its potential as a multifunctional biopolymer. Antimicrobial assays demonstrated significant activity against both Gram positive and Gram negative pathogens, including S. aureus, B. subtilis, E. coli, and S. typhi. Antioxidant assays confirmed its potent DPPH radical scavenging activity (71.52%) and Fe³⁺ reducing capacity. Furthermore, emulsification studies showed its efficacy with various oils (coconut oil, soybean oil, sunflower oil, olive oil) and a hydrocarbon (n-hexane), with the highest emulsification indices (E1 and E24) of 60.78% and 60.39%, respectively, against coconut and sunflower oils. Notably, the EPS selectively promoted the growth of probiotic strains while not enhancing the growth of pathogenic bacteria, highlighting its prebiotic potential.
These findings suggest that EPS from B. amyloliquefaciens BDIFST240014 is a promising candidate for diverse industrial and biomedical applications.
源自微生物的胞外多糖（EPS）因其生物可降解性和多样的生物活性，在各种工业应用中具有巨大潜力。
在本研究中，从解淀粉芽孢杆菌BDIFST240014中分离并表征了EPS，该革兰氏阳性菌以其强大的EPS生产能力而闻名。通过16S rRNA基因测序确认了分类学分类。我们优化了EPS生产的生长条件，在37°C孵育48小时后，最大产量约为74.81mg/L。使用紫外可见光谱和傅里叶变换红外光谱对EPS进行表征，揭示了表明其多糖性质的关键官能团。EPS的总碳水化合物和蛋白质含量分别为841.234μg/5mg和630.54μg/5mg，同时具有103.31%的强大持水能力（WHC）和106.78%的持油能力（OHC），突出了其作为多功能生物聚合物的潜力。抗菌试验表明对革兰氏阳性和革兰氏阴性病原体均具有显著活性，包括金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌和伤寒沙门氏菌。抗氧化试验证实了其强大的DPPH自由基清除活性（71.52%）和Fe³⁺还原能力。此外，乳化研究表明其对各种油（椰子油、大豆油、向日葵油、橄榄油）和一种烃（正己烷）具有乳化效果，对椰子油和向日葵油的最高乳化指数（E1和E24）分别为60.78%和60.39%。值得注意的是，EPS选择性地促进了益生菌菌株的生长，而不促进病原菌的生长，突出了其益生元潜力。
这些发现表明，解淀粉芽孢杆菌BDIFST240014产生的EPS是多种工业和生物医学应用的有前途的候选物。
