The Key Laboratory of Industrial Biotechnology, Ministry of Education , Jiangnan University , 1800 Lihu Road , Wuxi 214122 , P. R. China.
National Engineering Laboratory for Cereal Fermentation Technology , Jiangnan University , 1800 Lihu Road , Wuxi 214122 , P. R. China.
J Agric Food Chem. 2019 Jul 17;67(28):8029-8034. doi: 10.1021/acs.jafc.9b03234. Epub 2019 Jul 9.
A special levan-type exopolysaccharide (EPS) from JN4 with antiadhesive activity against enterotoxigenic (ETEC) was purified and identified. Chemical analysis indicated that EPS-JN4 with a low molecular weight of 8 kDa is composed of fructose and glucose with a molar ratio of 46.1:1. Structural analysis clarified that EPS-JN4 contains a main chain of β-(2,6)-linked Fru residues and intensive branches of a single 2-linked Fru at every six residues. Furthermore, the superior antiadhesive activity of EPS-JN4 against ETEC showed its potential usage as an antiadhesive agent for diarrhea prevention. EPS-JN4 is a specific type of levan family, for its small molecular size and intensive branches. The results expand the knowledge on structural types of levan and illustrate its potential as an antiadhesive agent for diarrhea prevention, which will be conducive to elucidate the relation between structure and function.
一株具有抗肠毒素性大肠杆菌（ETEC）黏附活性的特殊莱鲍迪苷型胞外多糖（EPS）JN4 被分离和鉴定。化学分析表明，相对分子质量为 8 kDa 的 EPS-JN4 由果糖和葡萄糖组成，摩尔比为 46.1:1。结构分析表明，EPS-JN4 含有β-（2,6）-连接的 Fru 残基主链和每隔六个残基的单一 2-连接 Fru 的密集支链。此外，EPS-JN4 对 ETEC 的优异抗黏附活性表明其有望用作预防腹泻的抗黏附剂。EPS-JN4 是一种特殊类型的莱鲍迪苷家族，因其分子量小且支链密集。该结果扩展了莱鲍迪苷结构类型的知识，并说明了其作为预防腹泻的抗黏附剂的潜力，这将有助于阐明结构与功能之间的关系。
