Tanış Ayşe Nur, Keskin İsmail
Faculty of Agriculture, Selçuk University, Konya, Turkey.
Mol Biol Rep. 2024 Dec 16;52(1):54. doi: 10.1007/s11033-024-10126-2.
There is a strong relationship between growth traits and genes. Identifying genotypes associated with superior phenotypic traits allows for targeted selection, facilitating the development of herds with desirable features. The growth hormone (GH) gene plays a crucial role in growth, development, and metabolism, making it a key focus for growth trait studies in livestock.
This study investigated the relationship between polymorphism in the GH gene and growth traits in 33 Akkaraman and 40 Anatolian Merino ewes. Traits such as birth weight (BW), weaning weight (WW), and 6-month weight (LW6), as well as body measurements (withers height (WH), rump height (RH), body length (BL), chest width (CW), chest circumference (CC)), were collected. Average daily weight gain (ADWG) and Kleiber ratios were calculated. Polymorphisms were detected through PCR-RFLP using the HaeIII enzyme, identifying three genotypes: AA, AB, and BB. Statistically significant differences were found between genotypes for WW, RH, WH, BL, CW, and CC (P < 0.05). Sheep with the AA genotype had higher averages, while AB individuals exhibited lower values for these traits. ADWG1 and KR1 values were higher in homozygous genotypes (AA and BB) than in heterozygous ones (AB), showing statistical significance (P < 0.05).
These results suggest that the GH gene may serve as a candidate for Marker-Assisted Selection (MAS) in Akkaraman and Anatolian Merino sheep, particularly for growth-related traits.
生长性状与基因之间存在密切关系。识别与优良表型性状相关的基因型有助于进行定向选择，促进培育具有理想特征的畜群。生长激素（GH）基因在生长、发育和新陈代谢中起着关键作用，使其成为家畜生长性状研究的重点。
本研究调查了33只阿卡曼羊和40只安纳托利亚美利奴羊GH基因多态性与生长性状之间的关系。收集了出生体重（BW）、断奶体重（WW）、6月龄体重（LW6）等性状，以及体尺指标（肩高（WH）、臀高（RH）、体长（BL）、胸宽（CW）、胸围（CC））。计算了平均日增重（ADWG）和克莱伯比率。使用HaeIII酶通过PCR-RFLP检测多态性，鉴定出三种基因型：AA、AB和BB。在WW、RH、WH、BL、CW和CC的基因型之间发现了统计学上的显著差异（P < 0.05）。AA基因型的绵羊这些性状的平均值较高，而AB个体这些性状的值较低。纯合基因型（AA和BB）的ADWG1和KR1值高于杂合基因型（AB），具有统计学意义（P < 0.05）。
这些结果表明，GH基因可能作为阿卡曼羊和安纳托利亚美利奴羊标记辅助选择（MAS）的候选基因，特别是对于与生长相关的性状。
