BACKGROUND: There has been an increasing interest in the development and prevention of sports injuries from a complex dynamic systems perspective. From this perspective, injuries may occur following critical fluctuations in the psychophysiological state of an athlete. Our objective was to quantify these so-called Early Warning Signals (EWS) as a proof of concept to determine their explanatory performance for injuries. The sample consisted of 23 professional youth football (soccer) players. Self-reports of psychological and physiological factors as well as data from heart rate and GPS sensors were gathered on every training and match day over two competitive seasons, which resulted in an average of 339 observations per player (range = 155-430). We calculated the Dynamic Complexity (DC) index of these data, representing a metric of critical fluctuations. Next, we used this EWS to predict injuries (traumatic and overuse). RESULTS: Results showed a significant peak of DC in 30% of the incurred injuries, in the six data points (roughly one and a half weeks) before the injury. The warning signal exhibited a specificity of 95%, that is, correctly classifying non-injury instances. We followed up on this promising result with additional calculations to account for the naturally imbalanced data (fewer injuries than non-injuries). The relatively low F we obtained (0.08) suggests that the model's overall ability to discriminate between injuries and non-injuries is rather poor, due to the high false positive rate. CONCLUSION: By detecting critical fluctuations preceding one-third of the injuries, this study provided support for the complex systems theory of injuries. Furthermore, it suggests that increasing critical fluctuations may be seen as an EWS on which practitioners can intervene. Yet, the relatively high false positive rate on the entire data set, including periods without injuries, suggests critical fluctuations may also precede transitions to other (e.g., stronger) states. Future research should therefore dig deeper into the meaning of critical fluctuations in the psychophysiological states of athletes. KEY POINTS: Complex Systems Theory suggests that sports injuries may be preceded by a warning signal characterized by a short window of increased critical fluctuations. Results of the current study showed such increased critical fluctuations before 30% of the injuries. Across the entire data set, we also found a considerable number of critical fluctuations that were not followed by an injury, suggesting that the warning signal may also precede transitions to other (e.g., healthier) states. Increased critical fluctuations may be interpreted as a window of opportunity for the practitioner to launch timely and targeted interventions, and researchers should dig deeper into the meaning of such fluctuations.