Dawangpa Atchara, Chitta Pitaksin, Rodrigues Guilherme da Silva, Iadsee Nutta, Noronha Natália Y, Nonino Carla B, Bueno Júnior Carlos R, Sae-Lee Chanachai
Research Division, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Department of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
PLoS One. 2024 Dec 16;19(12):e0315250. doi: 10.1371/journal.pone.0315250. eCollection 2024.
This study examined the effects of a 14-week combined exercise program on blood DNA methylation (DNAm) and its potential biological pathways in normal-weight, overweight, and obese older women. A total of 41 participants were assessed at baseline, 7 weeks, and 14 weeks into the training. Their whole-blood DNAm profiles were measured using the Infinitum MethylationEPIC BeadChip, alongside physical and biochemical health evaluations. The results showed notable health improvements, with decreases in blood pressure and cholesterol levels in the overweight and obese groups. Blood triglycerides were reduced only in the overweight group. Physical performance also improved across all groups. At 14 weeks, 1,043 differentially methylated positions (DMPs) were identified, affecting 744 genes. The genes were linked to biological processes, such as cellular metabolism, with significant pathway enrichment related to oxidative phosphorylation and chemical carcinogenesis. Additionally, the overweight group experienced significant reductions in methylation levels at eight lipogenesis-related genes. Protein EpiScore analysis revealed decreased levels of CCL11, VEGFA, and NTRK3 proteins at 14 weeks compared to baseline. Despite these significant molecular changes, there was no observable difference in DNAm age after the intervention. This study highlights how combined exercise can modify DNAm patterns in older women, particularly in lipogenesis-related genes, but suggests that further research is needed to understand the full implications for biological ageing.
本研究考察了一项为期14周的联合运动计划对正常体重、超重和肥胖老年女性血液DNA甲基化（DNAm）及其潜在生物学途径的影响。共有41名参与者在训练开始时、第7周和第14周接受评估。使用Infinitum MethylationEPIC BeadChip测量她们的全血DNAm谱，并进行身体和生化健康评估。结果显示健康状况有显著改善，超重和肥胖组的血压和胆固醇水平下降。只有超重组的血液甘油三酯降低。所有组的身体机能也都有所改善。在第14周时，鉴定出1043个差异甲基化位点（DMP），影响744个基因。这些基因与细胞代谢等生物学过程相关，与氧化磷酸化和化学致癌作用相关的通路有显著富集。此外，超重组8个与脂肪生成相关基因的甲基化水平显著降低。蛋白质EpiScore分析显示，与基线相比，第14周时CCL11、VEGFA和NTRK3蛋白水平降低。尽管有这些显著的分子变化，但干预后DNAm年龄没有明显差异。本研究强调了联合运动如何改变老年女性的DNAm模式，特别是在与脂肪生成相关的基因中，但表明需要进一步研究以了解其对生物衰老的全部影响。
