Zhang Tianyi, Gong Changwei, Pu Jian, Peng Anchun, Yang Jizhi, Wang Xuegui
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.
College of Agriculture, Sichuan Agricultural University, Chengdu 611130, China.
J Agric Food Chem. 2025 Jan 8;73(1):237-248. doi: 10.1021/acs.jafc.4c08903. Epub 2024 Dec 16.
, an extremely destructive invasive species, has rapidly spread in China, with queens exhibiting chemical tolerance. In this study, bioassays were conducted on colonies collected in Nanchong, revealing that the LC value of flonicamid for queens (3.91 mg/L) was significantly higher than that for workers (1.07 mg/L). Comparative analysis of transcriptomes of workers and queens treated with flonicamid revealed that differentially expressed genes (DEGs) were significantly enriched in the metabolism of xenobiotics by cytochrome P450 and drug metabolism by cytochrome P450 pathways. Based on the screening of transcriptome data, might be involved in the detoxification metabolism of flonicamid in queens. After RNA interference, the sensitivity of queens to flonicamid was significantly increased by 30% in the treatment of the group. Furthermore, heterologous overexpression of in also significantly enhanced the tolerance against flonicamid. These results indicated that the overexpression of in the queen enhances the tolerance against flonicamid.
（原文中未明确提及的某种生物），一种极具破坏性的入侵物种，已在中国迅速蔓延，其蚁后表现出化学耐受性。在本研究中，对在南充采集的（该生物）群体进行了生物测定，结果表明，氟啶虫胺腈对蚁后的LC值（3.91毫克/升）显著高于工蚁（1.07毫克/升）。对用氟啶虫胺腈处理的工蚁和蚁后的转录组进行比较分析发现，差异表达基因（DEGs）在细胞色素P450介导的异源物质代谢和细胞色素P450介导的药物代谢途径中显著富集。基于转录组数据的筛选，（某个基因或物质）可能参与了蚁后对氟啶虫胺腈的解毒代谢。RNA干扰后，在（某个组）的处理中，蚁后对氟啶虫胺腈的敏感性显著提高了30%。此外，在（某个生物或细胞系）中异源过表达（某个基因或物质）也显著增强了对氟啶虫胺腈的耐受性。这些结果表明，蚁后中（某个基因或物质）的过表达增强了对氟啶虫胺腈的耐受性。
