He Chunsheng, Qiu Zhisong, Jin Feng, Weng Lifang, Chen Libin, Wang Lijuan, Jiang Sicong, Shi Jin
Department of Gastroenterology, Cangshan Hospital, The 900th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Fuzhou, People's Republic of China.
Division of Thoracic and Endocrine Surgery, University Hospitals and University of Geneva, Geneva 1211, Switzerland.
Biomed Mater. 2025 Jan 10;20(2). doi: 10.1088/1748-605X/ad9fc7.
This study presents a novel electrochemical immunosensor for the detection of pepsinogen I, a potential biomarker for gastric cancer, based on a unique PdAgPt/MoSnanocomposite. The key innovation lies in the synergistic combination of trimetallic PdAgPt nanoparticles with MoSnanoflowers, which has not been previously reported for pepsinogen I detection. This hybrid material demonstrates exceptional electron transfer properties and a significantly larger electroactive surface area compared to conventional materials. The optimized immunosensor exhibits superior performance metrics: a wide linear range of 0.5-200 ng mland an unprecedented low detection limit of 0.173 ng ml, surpassing existing detection methods. The sensor shows remarkable selectivity with interfering substances exhibiting relative responses below 5%, excellent reproducibility (RSD 3.8%), and outstanding stability (95.6% retention after 30 d). Analysis of spiked serum samples resulted in recoveries ranging from 96.8% to 104.5%, demonstrating the sensor's practical applicability for early gastric cancer screening. This work represents a significant advancement in developing rapid, sensitive, and cost-effective diagnostic tools for gastric cancer surveillance.
本研究基于一种独特的钯银铂/二硫化钼纳米复合材料，提出了一种用于检测胃蛋白酶原I（一种胃癌潜在生物标志物）的新型电化学免疫传感器。关键创新在于三金属钯银铂纳米颗粒与二硫化钼纳米花的协同组合，此前尚未见用于胃蛋白酶原I检测的报道。这种混合材料表现出优异的电子转移性能，与传统材料相比，具有显著更大的电活性表面积。优化后的免疫传感器展现出卓越的性能指标：线性范围宽达0.5 - 200 ng/ml，检测限低至0.173 ng/ml，前所未有的低，超越了现有检测方法。该传感器对干扰物质表现出显著的选择性，相对响应低于5%，具有出色的重现性（相对标准偏差3.8%）和稳定性（30天后保留率95.6%）。加标血清样本分析的回收率在96.8%至104.5%之间，证明了该传感器在早期胃癌筛查中的实际适用性。这项工作代表了在开发用于胃癌监测的快速、灵敏且经济高效的诊断工具方面的重大进展。
