Moutinho Sara, Peres Helena, Fontinha Filipa, Estevão-Rodrigues Tássia, Monroig Óscar, Magalhães Rui, Pulido-Rodríguez Lina, Parisi Giuliana, Oliva-Teles Aires
Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal; Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n, Ed. FC4, 4169-007 Porto, Portugal.
Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal; Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre s/n, Ed. FC4, 4169-007 Porto, Portugal.
Comp Biochem Physiol B Biochem Mol Biol. 2025 Feb-Mar;276:111059. doi: 10.1016/j.cbpb.2024.111059. Epub 2024 Dec 15.
Hermetia illucens larvae oil (HIO) is a promising new ingredient that can potentially be an alternative lipid source in aquafeeds. To assess its viability in gilthead seabream juvenile diets, a 10-week feeding trial was performed, and the effects on antioxidant, immune, and inflammatory responses were evaluated. Four diets were formulated to include HIO at increasing levels: 0, 4, 7.9, and 9.5 %, replacing a vegetable oil mix at 0, 42 %, 84 %, and 100 %, respectively. At the end of the trial, no significant changes were detected in the plasma immune humoral parameters, except for a linear increase in plasma peroxidase activity. Hepatic lipid peroxidation (LPO) remained unchanged, while the activity of antioxidant enzymes showed a linear increase corresponding to the level of dietary HIO inclusion. Fish fed the HIO diets exhibited lower intestinal LPO, and no differences between groups were observed in the activity of the oxidative stress-related enzymes. Regarding the inflammation-related genes, the different diets did not affect interleukin-1β and transforming growth factor β expressions in the intestine. In contrast, upregulation of tumor necrosis factor α and interleukin-10 was observed, being higher in fish fed the diet with total vegetable oil replacement than the others. In conclusion, these findings suggest that Hermetia illucens larvae oil can be included at levels up to 7.9 % of gilthead seabream juvenile diets without compromising their immune, antioxidant, and inflammatory responses while enhancing intestinal LPO.
黑水虻幼虫油（HIO）是一种很有前景的新成分，有可能成为水产饲料中替代脂质来源。为评估其在金头鲷幼鱼饲料中的可行性，进行了为期10周的饲养试验，并评估了其对抗氧化、免疫和炎症反应的影响。配制了四种饲料，其中HIO含量逐渐增加：0%、4%、7.9%和9.5%，分别替代了0%、42%、84%和100%的植物油混合物。试验结束时，血浆免疫体液参数未检测到显著变化，但血浆过氧化物酶活性呈线性增加。肝脏脂质过氧化（LPO）保持不变，而抗氧化酶的活性随着饲料中HIO添加水平的增加呈线性增加。喂食含HIO饲料的鱼肠道LPO较低，各实验组之间氧化应激相关酶的活性未观察到差异。关于炎症相关基因，不同饲料对肠道中白细胞介素-1β和转化生长因子β的表达没有影响。相反，观察到肿瘤坏死因子α和白细胞介素-10上调，在完全替代植物油的饲料组中的鱼上调程度高于其他组。总之，这些发现表明，黑水虻幼虫油可以添加到金头鲷幼鱼饲料中，添加水平最高可达7.9%，而不会损害其免疫、抗氧化和炎症反应，同时还可增强肠道LPO。
