Kwon Dong-Hyeok, Lim Byeonghwi, Lee Sung-Yeon, Won Sung-Ho, Jang Goo
Laboratory of Theriogenology, College of Veterinary Medicine, Seoul National University, Seoul 08826; BK21 FOUR Future Veterinary Medicine Leading Education and Research Center, Seoul National University, Seoul 08826; Comparative Medicine Disease Research Center, Seoul National University, Seoul 08826, Korea.
Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
BMB Rep. 2025 Feb;58(2):95-103. doi: 10.5483/BMBRep.2024-0141.
Understanding molecular characteristics and metabolic processes of the mammalian endometrium is crucial for advancing biological research, particularly in veterinary obstetrics and pathology. This study established and analyzed organoids from endometrial epithelial stem cells of five mammals with different placental types: cows (cotyledonary), dogs and cats (zonary), pigs (diffuse), and rats (discoid). Organoids from these five species were maintained for over 13 passages, frozen, and thawed. Pathological analysis confirmed that they retained characteristics of their original tissues. Furthermore, integrative transcriptome analysis of organoids and tissues from the five species highlighted key pathways such as PI3K-Akt signaling and extracellular matrix-receptor interaction known to be crucial in cancer research. Although genes associated with vascular smooth muscle contraction were downregulated, these organoids exhibited significant activities of genes involved in hormone metabolism. In conclusion, our study achieved stable establishment of endometrial organoids from five mammals with different placental types, offering foundational data for organoid research. In the future, these organoids are suitable models for investigating uterine physiology and diseases and for developing potential therapies. [BMB Reports 2025; 58(2): 95-103].
了解哺乳动物子宫内膜的分子特征和代谢过程对于推进生物学研究至关重要，尤其是在兽医产科学和病理学领域。本研究建立并分析了来自五种不同胎盘类型哺乳动物的子宫内膜上皮干细胞类器官：牛（子叶型）、狗和猫（带状）、猪（弥散型）和大鼠（盘状）。这五个物种的类器官维持了超过13代，经过冷冻和解冻。病理分析证实它们保留了原始组织的特征。此外，对这五个物种的类器官和组织进行的综合转录组分析突出了关键途径，如PI3K-Akt信号通路和细胞外基质-受体相互作用，这些途径在癌症研究中至关重要。尽管与血管平滑肌收缩相关的基因下调，但这些类器官表现出参与激素代谢的基因的显著活性。总之，我们的研究成功地从五种不同胎盘类型的哺乳动物中稳定建立了子宫内膜类器官，为类器官研究提供了基础数据。未来，这些类器官是研究子宫生理学和疾病以及开发潜在治疗方法的合适模型。[《BMB报告》2025年；58(2): 95-103]
