Beuter Leonie, Bourauel Christoph, Singer Lamia
Oral Technology, Dental School, Medical Faculty, University Hospital Bonn, Bonn, Germany.
Department of Orthodontics, Dental School, Medical Faculty, University Hospital Bonn, Bonn, Germany.
Sci Rep. 2024 Dec 16;14(1):30516. doi: 10.1038/s41598-024-83035-w.
Impression materials can harbour microorganisms from saliva and blood, posing cross-contamination risks. However, post-setting disinfection might compromise the dimensional accuracy and mechanical properties of alginates. Hence, it was the aim of this research to assess the detail reproduction, tear strength, elastic recovery, and surface quality of the gypsum model of newly developed dental alginates with inherent antimicrobial properties.
Three dental alginate groups with antimicrobial alterations were formulated. One group replaced water with 0.2% chlorhexidine solution (CHX group), while the other two utilized water-based extracts of Syzygium aromaticum (SA, clove) or Zingiber officinale (ZO, ginger) to reduce silver nitrate, resulting in two silver nanoparticles (AgNPs)/extract (clove or ginger) mixture solutions. These mixture solutions were employed for the preparation of dental alginate yielding the SA + AgNP and ZO + AgNPs groups. All modified groups were compared to an unmodified control group that used water for mixing. Elastic recovery, detail reproduction, and tear strength were assessed following the ISO 21563:2021 standard. The surface roughness of plaster models was analysed using the optical profilometer. Elastic recovery was assessed by applying and then releasing load on alginate specimens to measure their ability to recover from deformation. Detail reproduction was evaluated by observing the reproducibility of a 50 μm line in a metallic mold using a light microscope, while tear strength was determined by stretching the specimens until failure at a constant speed of 500 mm/min.
All tested groups exhibited elastic recovery values meeting ISO standards for hydrocolloid impression materials. Regarding detail reproduction, both the control and modified alginates successfully reproduced the 50-µm line without interruption in all specimens. Tear strength values for all tested groups remained within the acceptable documented ranges, surpassing the minimum requirement of 0.38 N/mm as mandated by ISO 21563:2021. The ZO + AgNPs (0.94 ± 0.17 N/mm) demonstrated significantly higher tear strength values and surface roughness values compared to the other tested groups.
Chlorhexidine, Syzygium aromaticum, and Zingiber officinale green-synthesized silver nanoparticles are promising, cost-effective alternatives for disinfecting alginate impressions without compromising performance. Green nanoparticle synthesis is a safe, efficient, and non-toxic method, potentially synergizing metal ions with plant extract.
印模材料可能携带来自唾液和血液的微生物,带来交叉污染风险。然而,凝固后消毒可能会损害藻酸盐的尺寸精度和机械性能。因此,本研究的目的是评估具有固有抗菌性能的新开发牙科藻酸盐石膏模型的细节再现性、撕裂强度、弹性恢复和表面质量。
配制了三组具有抗菌改变的牙科藻酸盐。一组用0.2%氯己定溶液代替水(CHX组),而另外两组使用丁香(SA,丁香)或生姜(ZO,姜)的水基提取物来还原硝酸银,得到两种银纳米颗粒(AgNPs)/提取物(丁香或姜)混合溶液。这些混合溶液用于制备牙科藻酸盐,得到SA + AgNP组和ZO + AgNPs组。将所有改性组与使用水混合的未改性对照组进行比较。按照ISO 21563:2021标准评估弹性恢复、细节再现性和撕裂强度。使用光学轮廓仪分析石膏模型的表面粗糙度。通过对藻酸盐标本施加然后释放载荷来评估弹性恢复,以测量其从变形中恢复的能力。通过使用光学显微镜观察金属模具中50μm线条的再现性来评估细节再现性,而通过以500mm/min的恒定速度拉伸标本直至破坏来确定撕裂强度。
所有测试组的弹性恢复值均符合水胶体印模材料的ISO标准。关于细节再现性,对照组和改性藻酸盐在所有标本中均成功再现了50μm的线条,没有中断。所有测试组的撕裂强度值均保持在可接受的记录范围内,超过了ISO 21563:2021规定的0.38N/mm的最低要求。与其他测试组相比,ZO + AgNPs组(0.94±0.17N/mm)的撕裂强度值和表面粗糙度值明显更高。
氯己定以及丁香和姜绿色合成的银纳米颗粒是有前景的、具有成本效益的替代方案,可用于在不影响性能的情况下对藻酸盐印模进行消毒。绿色纳米颗粒合成是一种安全、高效且无毒的方法,可能使金属离子与植物提取物产生协同作用。