Liu Wenzhi, Tian Huiwu, Ma Jie, Xue Mingyang, Zhou Yong, Li Mengmeng, Jiang Jingwen, Fan Yuding, Liu Mingdian
Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Animals (Basel). 2024 Nov 25;14(23):3386. doi: 10.3390/ani14233386.
Different viruses are abundant in aquatic ecosystems. There has been limited research on the viral communities in the upper reaches of the Yangtze River. Yellow catfish (), an important economic fish that is widely distributed in the upper reaches of the Yangtze River, was selected as the research object. Using RNA sequencing, we identified 11 viruses belonging to the , , , , , and other bacteriophage families. The predominant viral families/order in Luzhou (LZ), Fuling (FL), and Wanzhou (WZ) were , and , respectively. The virome from WZ had a unique community composition, with a high abundance of compared with LZ and FL. In LZ, the predominant double-stranded RNA virus family was . Phylogenetic analyses showed that viruses presented high genetic diversity. Phylogenetically, Wenling pleuronectiformes picornavirus was close to the family , which includes yellow catfish calicivirus (YcCV), responsible for the massive mortality of yellow catfish in 2020. This study provides insights into the viral community composition in yellow catfish in the upper reaches of the Yangtze River, revealing a diverse and unique river water virome and providing clues for future research on the origin of viral pathogens.
不同的病毒在水生生态系统中大量存在。对长江上游的病毒群落研究有限。黄颡鱼是一种重要的经济鱼类，广泛分布于长江上游，被选为研究对象。通过RNA测序，我们鉴定出11种属于、、、、、和其他噬菌体家族的病毒。泸州（LZ）、涪陵（FL）和万州（WZ）的主要病毒家族/目分别为、和。万州的病毒群落组成独特，与泸州和涪陵相比，的丰度较高。在泸州，主要的双链RNA病毒家族是。系统发育分析表明，病毒呈现出高度的遗传多样性。在系统发育上，温岭鲽形目小RNA病毒与包括黄颡鱼杯状病毒（YcCV）在内的科关系密切，黄颡鱼杯状病毒在2020年导致了黄颡鱼的大量死亡。本研究为长江上游黄颡鱼的病毒群落组成提供了见解，揭示了一个多样且独特的河水病毒群落，并为未来病毒病原体起源的研究提供了线索。
