Yang Dong, Ma Xiaowan, Zhong Shengping, Guo Jiasen, Cheng Dewei, Chen Xuyang, Huang Teng, Huang Lixing, Qiao Ying, Pengsakul Theerakamol
Guangxi University, Nanning 530200, China.
Key Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, China.
Animals (Basel). 2024 Nov 25;14(23):3401. doi: 10.3390/ani14233401.
infection, which causes visceral white spot disease, is a significant and economically devastating disease in aquaculture. In this study, we investigated the impact of bacterial infection on the protein composition of exosomes derived from the surface mucus of the hybrid grouper ♀ × ♂. Two hundred healthy fish were randomly separated into challenge and control groups. Fish from the challenge group received 10 CFU/g of the bacterial pathogen via intraperitoneal injection, while sterile PBS was used as a negative control. After injection, the mucus was collected and the exosomes were extracted for proteomic analysis. The results of proteomic analysis revealed that infection significantly increased the levels of innate immune proteins, including lysosomal and peroxisomal proteins, within the exosomes. Furthermore, the CAD protein was found to play a pivotal role in the protein interaction networks involved in the response to infection. Intriguingly, we also observed a significant increase in the levels of metal-binding proteins within the exosomes, providing important evidence of nutritional immunity on the surfaces of the fish hosts. Notably, several proteins, such as plasma kallikrein, Annexin A5, eukaryotic translation initiation factor 3 subunit M, and S-methyl-5-thioadenosine phosphorylase, exhibited a remarkable increase in abundance in exosomes after infection. These proteins show promising potential as noninvasive biomarkers for the diagnosis of visceral white spot disease. The study contributes to the understanding of the host response to infection and may aid policymakers in implementing appropriate intervention measures for effective risk management of this devastating disease.
导致内脏白点病的感染是水产养殖中一种严重且具有经济破坏性的疾病。在本研究中，我们调查了细菌感染对杂交石斑鱼♀×♂体表黏液来源外泌体蛋白质组成的影响。将200条健康鱼随机分为感染组和对照组。感染组鱼通过腹腔注射接种10 CFU/g的细菌病原体，而无菌磷酸盐缓冲盐水用作阴性对照。注射后，收集黏液并提取外泌体进行蛋白质组学分析。蛋白质组学分析结果显示，感染显著增加了外泌体内先天免疫蛋白的水平，包括溶酶体蛋白和过氧化物酶体蛋白。此外，发现CAD蛋白在参与应对感染的蛋白质相互作用网络中起关键作用。有趣的是，我们还观察到外泌体内金属结合蛋白水平显著增加，这为鱼类宿主表面的营养免疫提供了重要证据。值得注意的是，一些蛋白质，如血浆激肽释放酶、膜联蛋白A5、真核翻译起始因子3亚基M和S-甲基-5'-硫代腺苷磷酸化酶，在感染后的外泌体中丰度显著增加。这些蛋白质作为内脏白点病诊断的非侵入性生物标志物具有广阔的潜力。该研究有助于理解宿主对感染的反应，并可能帮助政策制定者实施适当的干预措施，以有效管理这种毁灭性疾病的风险。
