Gradner Gabriele, Janssen Janina, Oevermann Anna, Tichy Alexander, Kummer Stefan, Burger Stefanie, Walter Ingrid
Small Animal Surgery Unit, University of Veterinary Medicine, 1210 Veterinaerplatz 1, 1210 Vienna, Austria.
Department of Clinical Research and Veterinary Public Health, University of Veterinary Medicine, 3001 Bremgartenstrasse 109 a, 3014 Bern, Switzerland.
Animals (Basel). 2024 Nov 26;14(23):3404. doi: 10.3390/ani14233404.
The high recurrence rate of feline meningioma despite the generally benign histomorphology warrants additional markers of clinical aggressiveness. The Ki-67 index is commonly used as prognostic marker for meningioma recurrence in people. Osteopontin (OPN) is a protein involved in tumor progression and may be a potential malignancy marker. To date, osteopontin expression has not been investigated in feline meningioma. The aim of this study was to evaluate the extent of Ki-67 and osteopontin immunostaining of feline meningioma and to find possible associations with WHO (World Health Organization) grades and subtypes. Fifty-three feline meningioma samples were graded according to the human WHO classification and underwent immunohistochemical examination for Ki-67 and OPN. Fifty samples were classified as WHO grade I and three as WHO grade II. The mean Ki-67 ratio was 9.19 ± 9.47. Osteopontin expression was correspondingly high with a mean OPN IHC score of 150.17 (0-242.8), and a median Allred score of 7 (0-8). There was no significant correlation with Ki-67 index, osteopontin expression, WHO grades, or subtypes. The overall high expressions of osteopontin and Ki-67 may help explain the tendency for recurrence of feline meningioma. The human WHO grading system may not be sufficient to accurately estimate the clinical behavior of meningioma in this species.
尽管猫脑膜瘤的组织形态学通常为良性，但其高复发率仍需要更多临床侵袭性标志物。Ki-67指数通常用作人类脑膜瘤复发的预后标志物。骨桥蛋白（OPN）是一种参与肿瘤进展的蛋白质，可能是一种潜在的恶性标志物。迄今为止，尚未对猫脑膜瘤中的骨桥蛋白表达进行研究。本研究的目的是评估猫脑膜瘤中Ki-67和骨桥蛋白免疫染色的程度，并寻找与世界卫生组织（WHO）分级和亚型的可能关联。根据人类WHO分类对53例猫脑膜瘤样本进行分级，并对Ki-67和OPN进行免疫组化检查。50个样本被分类为WHO I级，3个为WHO II级。Ki-67平均比率为9.19±9.47。骨桥蛋白表达相应较高，平均OPN免疫组化评分为150.17（0-242.8），Allred评分中位数为7（0-8）。与Ki-67指数、骨桥蛋白表达、WHO分级或亚型均无显著相关性。骨桥蛋白和Ki-67的总体高表达可能有助于解释猫脑膜瘤的复发倾向。人类WHO分级系统可能不足以准确估计该物种脑膜瘤的临床行为。
