Thompson G C, Igarashi M, Stach B A
J Comp Neurol. 1985 Jan 8;231(2):270-9. doi: 10.1002/cne.902310214.
The location of brainstem neurons which mediate the stapedius reflex was identified by injecting horseradish peroxidase into the stapedius muscle of squirrel monkeys and bush babies. Retrogradely labeled neurons, arranged in a one- to three-cell column, were found medial to the main facial motor nucleus in squirrel monkeys and ventral to it in bush babies. Nissl, protargol, and acetylcholinesterase stains were subsequently used to identify and describe this unique column of cells. It was found that staining characteristics, as well as shape, size, and location, distinguish stapedius muscle motoneurons from closely associated cell groups. Furthermore, stapedius muscle motoneurons are morphologically similar to periolivary cells and morphologically dissimilar to cells within the facial motor nucleus.
通过将辣根过氧化物酶注入松鼠猴和丛猴的镫骨肌中，确定了介导镫骨肌反射的脑干神经元的位置。在松鼠猴中，逆行标记的神经元排列成一到三个细胞的柱状，位于面神经主运动核的内侧；在丛猴中，则位于其腹侧。随后使用尼氏染色、原银染色和乙酰胆碱酯酶染色来识别和描述这一独特的细胞柱。研究发现，染色特征以及形状、大小和位置，可将镫骨肌运动神经元与紧密相关的细胞群区分开来。此外，镫骨肌运动神经元在形态上与橄榄周细胞相似，而与面神经运动核内的细胞形态不同。
