肝脏再生潜能临界下降的新型光学标准及机制

Novel Optical Criteria and Mechanisms of Critical Decline in Liver Regenerative Potential.

作者信息

Rodimova Svetlana, Kozlova Vera, Bobrov Nikolai, Kozlov Dmitry, Mozherov Artem, Elagin Vadim, Shchechkin Ilya, Kuzmin Dmitry, Gavrina Alena, Zagainov Vladimir, Zagaynova Elena, Kuznetsova Daria

机构信息

Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia.

Department of Molecular Biology and Immunology, Lobachevsky Nizhny Novgorod National Research State University, Gagarina 23, 603022 Nizhny Novgorod, Russia.

出版信息

Cells. 2024 Dec 5;13(23):2015. doi: 10.3390/cells13232015.

DOI:10.3390/cells13232015

PMID:39682763

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11639982/

Abstract

The most effective method of treating tumors localized in the liver remains resection. However, in the presence of concomitant pathology, the regenerative potential of the liver is significantly reduced. To date, there is insufficient fundamental data on the mechanisms responsible for the disruption of liver regeneration, and there is no effective method for assessing its regenerative potential. The most suitable model for these purposes is acute liver injury (ALI). Modern non-contrast methods of multiphoton microscopy with second harmonic generation and fluorescence lifetime imaging microscopy (FLIM) modes enable intravital evaluation of the metabolic status of the hepatocytes; therefore, this expands the possibilities for studying the processes occurring in cells during regeneration in the context of any pathologies.

摘要

治疗局限于肝脏的肿瘤最有效的方法仍然是切除。然而，在存在伴随病变的情况下，肝脏的再生潜力会显著降低。迄今为止，关于肝脏再生破坏机制的基础数据不足，且尚无评估其再生潜力的有效方法。急性肝损伤（ALI）是用于这些目的的最合适模型。具有二次谐波产生和荧光寿命成像显微镜（FLIM）模式的现代多光子显微镜非对比方法能够对肝细胞的代谢状态进行活体评估；因此，这扩展了在任何病理情况下研究再生过程中细胞内发生的过程的可能性。