Ferri Irene, Spano Mattia, Dell'Anno Matteo, Mannina Luisa, Rossi Luciana
Department of Veterinary Medicine and Animal Sciences-DIVAS, University of Milan, Via dell'Università 6, 26900 Lodi, Italy.
Food Chemistry Lab, Department of Chemistry and Technology of Drugs, Sapienza University of Rome, 00185 Rome, Italy.
Foods. 2024 Nov 24;13(23):3757. doi: 10.3390/foods13233757.
The aim of this study was to evaluate the metabolomic profile of larvae reared on the following innovative growth substrates: wheat bran (control, CTRL); wheat bran supplemented with 12.5% / chestnut shell (TRT1); and wheat bran supplemented with 25% / chestnut shell (TRT2) for 14 days of trial. At the end of this experiment, larvae were transformed into insect meals for nutritional characterization. Nuclear Magnetic Resonance (NMR) spectroscopy was carried out to evaluate the metabolomic profile of organic acids, sugars, nitrogen bases and derivates, fatty acids, and other compounds. Chemical analysis showed an increased level of crude protein in TRT1 compared to CTRL and TRT2 ( 0.0391). The metabolite profiles of TRT1 and TRT2 were similar to each other but distinct from those of the CTRL group. Notably, larvae enriched with chestnut shells revealed the presence of uracil, uridine, and glucose, while fumarate was absent. The enrichment analysis showed that in TRT1 and TRT2, the glyoxylate and dicarboxylate metabolism was more relevant compared to CTRL. These findings indicate that chestnut shell inclusion affects the larvae metabolism of and demonstrates the effectiveness of NMR spectroscopy in revealing a relation between insect metabolism and growth substrate.
麦麸（对照组，CTRL）；添加12.5%栗壳的麦麸（TRT1）；以及添加25%栗壳的麦麸（TRT2），试验为期14天。在该实验结束时，将幼虫转化为昆虫粉进行营养特性分析。采用核磁共振（NMR）光谱法评估有机酸、糖类、含氮碱基及其衍生物、脂肪酸和其他化合物的代谢组学特征。化学分析表明，与CTRL和TRT2相比，TRT1中的粗蛋白水平有所提高（0.0391）。TRT1和TRT2的代谢物谱彼此相似，但与CTRL组不同。值得注意的是，富含栗壳的幼虫中存在尿嘧啶、尿苷和葡萄糖，而富马酸盐不存在。富集分析表明，与CTRL相比，TRT1和TRT2中的乙醛酸和二羧酸代谢更为相关。这些发现表明，添加栗壳会影响幼虫的代谢，并证明了NMR光谱法在揭示昆虫代谢与生长基质之间关系方面的有效性。
