Ferri Irene, Dell'Anno Matteo, Spano Mattia, Canala Benedetta, Petrali Beatrice, Dametti Matilda, Magnaghi Stefano, Rossi Luciana
Department of Veterinary Medicine and Animal Sciences-DIVAS, University of Milan, 26900 Lodi, Italy.
Food Chemistry Lab, Department of Chemistry and Technology of Drugs, Sapienza University of Rome, 00185 Rome, Italy.
Insects. 2024 Jul 9;15(7):512. doi: 10.3390/insects15070512.
larvae represent a sustainable protein source for food and feed. The aim of this study was to evaluate the supplementation of chestnut shell, a by-product of the agro-industrial chain, in growth substrates for larvae rearing. Seven-week-old larvae were reared on three different growth substrates: the control group (CTRL) was fed wheat bran, treatment group one was fed wheat bran supplemented with 12.5% / chestnut shell (TRT1), and treatment group two was fed wheat bran supplemented with 25% / chestnut shell (TRT2). Larval weight, substrate consumption, and mortality were recorded weekly. After 14 days, insect meals were produced for bromatological and colorimetric analysis, and bacterial inhibition activity assay using a microdilution method. The amino acid profile of insects was determined using quantitative nuclear magnetic resonance spectroscopy. Our results showed a lower feed conversion ratio and higher larval survival rate % in TRT2 compared to CTRL ( < 0.05). Proteins and lipids of TRT2 were higher than other groups ( < 0.05). Important differences were observed in the amino acid profile of TRT1 and TRT2 compared to CTRL ( < 0.05). TRT1 and TRT2 showed higher inhibitory activity than CTRL ( < 0.05). In conclusion, chestnut shell supplementation improved the survival and functional characteristics of larvae and likely impacted the insects' metabolism.
幼虫是食品和饲料的可持续蛋白质来源。本研究的目的是评估农业产业链副产品栗壳在幼虫饲养生长基质中的添加效果。将7周龄的幼虫饲养在三种不同的生长基质上：对照组（CTRL）喂食麦麸，处理组一喂食添加12.5%栗壳的麦麸（TRT1），处理组二喂食添加25%栗壳的麦麸（TRT2）。每周记录幼虫体重、基质消耗和死亡率。14天后，制备昆虫粉用于进行食品分析、比色分析以及采用微量稀释法进行细菌抑制活性测定。使用定量核磁共振光谱法测定昆虫的氨基酸谱。我们的结果表明，与对照组相比，TRT2的饲料转化率较低，幼虫存活率较高（<0.05）。TRT2的蛋白质和脂质含量高于其他组（<0.05）。与对照组相比，TRT1和TRT2的氨基酸谱存在重要差异（<0.05）。TRT1和TRT2显示出比对照组更高的抑制活性（<0.05）。总之，添加栗壳改善了幼虫的存活率和功能特性，并可能影响了昆虫的新陈代谢。
