Kim Jinhee, Lee Minhee, Cho Wonhee, Yoo Eunhee, Kim Jinhak, Gwon Yuri, Okayasu Musashi, Lee Jeongmin
Department of Medical Nutrition, Kyung Hee University, Yongin 17104, Republic of Korea.
Department of Food Innovation and Health, Kyung Hee University, Yongin 17104, Republic of Korea.
Foods. 2024 Nov 27;13(23):3824. doi: 10.3390/foods13233824.
This study investigated the protective effects of a ceramides derivates from the peach (PF3) on photoaging by UV-irradiated hairless mice. Mice were randomly divided into seven groups: AIN93G without UVB exposure (normal control, NC), AIN93G with UVB exposure (control, C), AIN93G supplemented 100 mg/kg body weight (BW) of L-ascorbic acid with UVB exposure (AA), AIN93G supplemented 100 mg/kg BW of arbutin with UVB exposure (Arbutin), AIN93G supplemented 10 mg/kg BW of PF3 with UVB exposure (10PF3), AIN93G supplemented 20 mg/kg BW of PF3 with UVB exposure (20PF3), and AIN93G supplemented 40 mg/kg BW of PF3 with UVB exposure (40PF3). The study examined the impact of PF3 on skin hydration, wrinkle formation, and melanogenesis using enzyme-linked immunosorbent assay (ELISA), real-time polymerase chain reaction (real-time PCR), and Western blot analysis. The PF3 demonstrated significant protective effects against photoaging by reducing skin wrinkle formation, decreasing epidermal and dermal thickening, and improving skin hydration. It also enhanced the expression of moisture-related factors (hyaluronic acid synthase [HAS], long-chain ceramides [LCBs], dihydroceramide desaturase 1 [DEGS1], and type I collagen [COL1A]) and antioxidant enzyme activities while reducing pro-inflammatory cytokines and oxidative stress markers. The PF3 supplementation positively modulated skin wrinkle formation-related factors, increasing collagen-related gene expression and decreasing matrix metalloproteinases. Additionally, PF3 showed potential in regulating melanogenesis by reducing the nitric oxide and cAMP content, as well as the expression of melanogenesis-related proteins. These comprehensive findings suggest that PF3 supplementation may be an effective strategy for preventing and treating UVB-induced skin photoaging through multiple mechanisms, including improved skin structure, hydration, antioxidant defense, and reduced inflammation and pigmentation.
本研究调查了桃来源的神经酰胺衍生物（PF3）对紫外线照射的无毛小鼠光老化的保护作用。小鼠被随机分为七组：未接受UVB照射的AIN93G（正常对照，NC）、接受UVB照射的AIN93G（对照，C）、接受UVB照射且补充100 mg/kg体重（BW）L-抗坏血酸的AIN93G（AA）、接受UVB照射且补充100 mg/kg BW熊果苷的AIN93G（熊果苷）、接受UVB照射且补充10 mg/kg BW PF3的AIN93G（10PF3）、接受UVB照射且补充20 mg/kg BW PF3的AIN93G（20PF3）以及接受UVB照射且补充40 mg/kg BW PF3的AIN93G（40PF3）。该研究使用酶联免疫吸附测定（ELISA）、实时聚合酶链反应（实时PCR）和蛋白质印迹分析，检测了PF3对皮肤水合作用、皱纹形成和黑色素生成的影响。PF3通过减少皮肤皱纹形成、降低表皮和真皮增厚以及改善皮肤水合作用，对光老化表现出显著的保护作用。它还增强了与水分相关因子（透明质酸合酶[HAS]、长链神经酰胺[LCB]、二氢神经酰胺去饱和酶1[DEGS1]和I型胶原蛋白[COL1A]）的表达以及抗氧化酶活性，同时减少促炎细胞因子和氧化应激标志物。补充PF3对皮肤皱纹形成相关因子产生积极调节作用，增加胶原蛋白相关基因表达并降低基质金属蛋白酶。此外，PF3通过降低一氧化氮和cAMP含量以及黑色素生成相关蛋白的表达，在调节黑色素生成方面显示出潜力。这些综合研究结果表明，补充PF3可能是一种通过多种机制预防和治疗UVB诱导的皮肤光老化的有效策略，这些机制包括改善皮肤结构、水合作用、抗氧化防御以及减少炎症和色素沉着。
