Qiu Chen, Jin Xiaochen, Zhao Yumiao, Kuai Peng, Lou Yonggen
State Key Laboratory of Rice Biology & Ministry of Agriculture Key Laboratory of Agricultural Entomology, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
Hainan Institute, Zhejiang University, Sanya 572025, China.
Plants (Basel). 2024 Nov 22;13(23):3275. doi: 10.3390/plants13233275.
Plant nucleotide-binding leucine-rich repeat immune receptor genes (NLRs) play an important role in plant defenses against pathogens, pathogenic nematodes, and piercing-sucking herbivores. However, little is known about their functions in plant defenses against chewing herbivores. Here, we identified a plasma membrane-localized coiled-coil-type NLR protein, OsPik-2-like, whose transcript levels were induced by the infestation of rice leaf folder (LF, ) larvae, and by treatment with mechanical wounding. Knocking out in rice increased the LF-induced levels of jasmonic acid (JA) and jasmonoyl-isoleucine (JA-Ile), the activity of trypsin protease inhibitors (TrypPIs), and the basal levels of some flavonoids, which in turn decreased the performance of LF larvae. Moreover, knocking out reduced plant growth. These findings demonstrate that OsPik-2-like regulates the symbiosis between rice and LF by balancing plant growth and defense.
植物核苷酸结合富含亮氨酸重复序列免疫受体基因（NLRs）在植物抵御病原体、病原线虫和刺吸式食草动物中发挥重要作用。然而，它们在植物抵御咀嚼式食草动物中的功能却鲜为人知。在此，我们鉴定出一种定位于质膜的卷曲螺旋型NLR蛋白，即类OsPik-2，其转录水平受稻纵卷叶螟（LF）幼虫侵染以及机械损伤处理诱导。敲除水稻中的 会增加LF诱导的茉莉酸（JA）和茉莉酰异亮氨酸（JA-Ile）水平、胰蛋白酶蛋白酶抑制剂（TrypPIs）的活性以及一些黄酮类化合物的基础水平，进而降低LF幼虫的生长性能。此外，敲除 会降低植物生长。这些发现表明，类OsPik-2通过平衡植物生长和防御来调节水稻与LF之间的共生关系。
