Karahüseyin Seçil, Özsoy Nurten, Özbek Çelik Berna, Sarı Aynur
Department of Pharmacognosy, Faculty of Pharmacy, Cukurova University, 01330 Adana, Türkiye.
Department of Pharmacognosy, Institute of Health Sciences, Istanbul University, 34116 Istanbul, Türkiye.
Plants (Basel). 2024 Nov 25;13(23):3304. doi: 10.3390/plants13233304.
In this study, the aim was to investigate the chemical content and in vitro antioxidant, antimicrobial, and anti-inflammatory activities of petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EA), and n-butanol (n-BuOH) fractions obtained from ethanol extracts of the aerial parts and roots of the endemic Wagenitz. This plant is found in the Aksaray-Eskil region and has not been studied in phytochemical studies before. In this context, the chemical content of the aerial parts and root PE fractions was analyzed by GC-MS analysis in terms of terpenes and steroid substances. The composition of phenolic compounds in the aerial parts and root DCM and EA fractions was determined by HPLC-MS analysis. Apigenin, luteolin, and caffeic acid were isolated from the EA fraction of aerial parts. The total amounts of phenolic substances and the DPPH, ABTS, and FRAP antioxidant activities of PE, DCM, EA, and n-BuOH fractions were investigated, and it was found that the fractions had the ability to scavenge DPPH and ABTS radicals, as well as to reduce Fe (III) to Fe (II); however, all of the fractions were significantly less effective ( < 0.05) than the reference antioxidant quercetin. Considering that antioxidants can also exert an anti-inflammatory effect, these fractions were evaluated for their ability to inhibit cyclooxygenases (COX-1 and COX-2), the key enzymes of arachidonic acid metabolism that lead to the production of important mediators of inflammation. It was observed that fractions had the ability to inhibit both enzymes, suggesting their possible beneficial effects against inflammation. However, no extract had greater inhibitory activity than the positive control, indomethacin. The antimicrobial activity was determined against different bacterial and fungal strains. It was observed that the aerial parts and root n-BuOH and EA fractions showed weak antibacterial effects. No antifungal activity has been detected against sp.
在本研究中，目的是调查从地方特有植物瓦格尼茨（Wagenitz）地上部分和根部的乙醇提取物中获得的石油醚（PE）、二氯甲烷（DCM）、乙酸乙酯（EA）和正丁醇（n-BuOH）馏分的化学成分以及体外抗氧化、抗菌和抗炎活性。这种植物生长在阿克萨赖-埃斯基尔地区，之前在植物化学研究中尚未被研究过。在此背景下，通过气相色谱-质谱联用（GC-MS）分析对地上部分和根部PE馏分中的萜类和甾体物质的化学成分进行了分析。通过高效液相色谱-质谱联用（HPLC-MS）分析确定了地上部分以及根部DCM和EA馏分中酚类化合物的组成。芹菜素、木犀草素和咖啡酸从地上部分的EA馏分中分离出来。研究了PE、DCM、EA和n-BuOH馏分中酚类物质的总量以及二苯基苦味酰基自由基（DPPH）、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和铁离子还原抗氧化能力（FRAP）抗氧化活性，发现这些馏分具有清除DPPH和ABTS自由基以及将三价铁（Fe(III)）还原为二价铁（Fe(II)）的能力；然而，所有馏分的效果均明显低于参考抗氧化剂槲皮素（<0.05）。考虑到抗氧化剂也可发挥抗炎作用，对这些馏分抑制环氧合酶（COX-1和COX-2）的能力进行了评估，环氧合酶是花生四烯酸代谢的关键酶，可导致产生重要的炎症介质。观察到馏分具有抑制这两种酶的能力，表明它们可能对炎症具有有益作用。然而，没有提取物具有比阳性对照吲哚美辛更强的抑制活性。针对不同的细菌和真菌菌株测定了抗菌活性。观察到地上部分以及根部的n-BuOH和EA馏分显示出较弱的抗菌作用。未检测到对 种的抗真菌活性。
