通过 UPLC/ESI/TOF-MS 分析埃及芹菜（Apiaceae）地上部分多酚富集提取物的抗氧化、抗菌活性及特性。
Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery ( L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MS.
机构信息
Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Sixth of October City 12511, Egypt.
Microbiology & Immunology Department, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, Egypt.
出版信息
Molecules. 2022 Jan 21;27(3):698. doi: 10.3390/molecules27030698.
Medicinal plant extracts are increasingly considered a major source of innovative medications and healthcare products. This study focused on preparing a polyphenol enriched water extract of Egyptian celery " L., Apiaceae" aerial parts (TAE) in an endeavor to accentuate its antioxidant capacity as well as its antimicrobial activity. (TAE) of celery was partitioned against different organic solvents to yield dichloromethane (DCM), ethyl acetate (EAC), and butanol (BUOH) fractions. (TAE) and the organic fractions thereof besides the remaining mother liquor (ML) were all screened for their antioxidant capacity using various protocols viz. monitoring the reducing amplitudes for ferric ions (FRAP), and radical scavenging potentials of oxygen (ORAC), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and metal chelation assays. The examination procedure revealed both (TAE) extract and (DCM) fraction, to pertain the highest antioxidant potentials, where the IC of the (TAE) using ABTS and metal chelation assays were ca. 34.52 ± 3.25 and 246.6 ± 5.78 µg/mL, respectively. The (DCM) fraction recorded effective results using the FRAP, ORAC, and DPPH assays ca. 233.47 ± 15.14 and 1076 ± 25.73 μM Trolox equivalents/mg sample and an IC 474.4 ± 19.8 µg/mL, respectively. Additionally, both (TAE) and (DCM) fraction exerted antimicrobial activities recording inhibition zones (mm) (13.4 ± 1.5) and (12.0 ± 1.0) against and (11.0 ± 1.2) and (10.0 ± 1.3) against , respectively, with no anti-fungal activity. Minimum inhibitory concentration (MIC) of (TAE) and (DCM) fraction were 1250 and 2500 µg/mL, respectively. UPLC/ESI/TOF-MS unveiled the chemical profile of both (TAE) and (DCM) fraction to encompass a myriad of active polyphenolic constituents including phenylpropanoids, coumarins, apigenin, luteolin, and chrysoeriol conjugates.
药用植物提取物越来越被认为是创新药物和医疗保健产品的主要来源。本研究旨在制备富含多酚的埃及芹菜 "L.，伞形科 "地上部分（TAE）的水提取物，以强调其抗氧化能力和抗菌活性。将芹菜的（TAE）用不同的有机溶剂萃取，得到二氯甲烷（DCM）、乙酸乙酯（EAC）和正丁醇（BUOH）级分。（TAE）及其有机级分以及剩余的母液（ML）均根据各种方案筛选其抗氧化能力，即监测铁离子的还原幅度（FRAP）、氧自由基清除能力（ORAC）、2,2'-偶氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）、2,2-二苯基-1-苦基肼（DPPH）和金属螯合测定。检查程序表明，（TAE）提取物和（DCM）级分都具有最高的抗氧化潜力，（TAE）使用 ABTS 和金属螯合测定的 IC 分别约为 34.52 ± 3.25 和 246.6 ± 5.78 µg/mL。（DCM）级分在 FRAP、ORAC 和 DPPH 测定中记录了有效的结果，分别为约 233.47 ± 15.14 和 1076 ± 25.73 μM Trolox 当量/mg 样品和 IC 474.4 ± 19.8 µg/mL。此外，（TAE）和（DCM）级分均表现出抗菌活性，对 和 分别记录了 13.4 ± 1.5 和 12.0 ± 1.0 毫米的抑菌圈，对 和 分别记录了 11.0 ± 1.2 和 10.0 ± 1.3 毫米的抑菌圈，没有抗真菌活性。（TAE）和（DCM）级分的最小抑菌浓度（MIC）分别为 1250 和 2500 µg/mL。UPLC/ESI/TOF-MS 揭示了（TAE）和（DCM）级分的化学特征，其中包含多种活性多酚成分，包括苯丙素类、香豆素、芹菜素、木犀草素和 chrysoeriol 缀合物。
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