Omari Alzahrani Fatima
Department of Biology, Faculty of Sciences, Al-Baha University, Al-Baha 65729, Saudi Arabia.
Plants (Basel). 2024 Nov 27;13(23):3327. doi: 10.3390/plants13233327.
Barley ( L.) is among the earliest crops to be cultivated and is also considered a crucial staple crop. Nevertheless, the negative effects of abiotic stress on both the quality and productivity of barley are significant. Nine-cis-epoxycarotenoid dioxygenases (NCEDs) are rate-limiting enzymes in plants that cleave carotenoids and produce abscisic acid (ABA). The poor utilization of barley s in stress-resistant genetic breeding is due to the lack of appropriate information about their potential function in abiotic stress. The current study revealed five genes in the barley genome (-), which are distributed unevenly on barley chromosomes. The PF03055 domain is present in all s, and they encode 413~643 amino acids. Phylogenetic analysis showed that genes were categorized into three distinct clades, confirming the homology of genes between L., L., and L. Expression analysis revealed that 1 is significantly upregulated under high salt stress, indicating its potential role in enhancing salt tolerance. In contrast, 3 and 4 exhibited downregulation, suggesting a complex regulatory mechanism in response to varying salt stress levels. These findings will enhance our comprehension of the genetic composition and evolutionary development of the gene family and provide a basis for future research on their role in response to salt-induced stress.
大麦（L.）是最早被栽培的作物之一，也被认为是一种重要的主粮作物。然而，非生物胁迫对大麦的品质和产量都有显著的负面影响。九顺式环氧类胡萝卜素双加氧酶（NCEDs）是植物中裂解类胡萝卜素并产生脱落酸（ABA）的限速酶。大麦在抗逆遗传育种中的利用不足，是由于缺乏关于其在非生物胁迫中潜在功能的适当信息。当前的研究揭示了大麦基因组中的五个基因（-），它们在大麦染色体上分布不均。所有基因中都存在PF03055结构域，并且它们编码413至643个氨基酸。系统发育分析表明，这些基因被分为三个不同的进化枝，证实了大麦（L.）、小麦（L.）和黑麦（L.）之间这些基因的同源性。表达分析显示，1在高盐胁迫下显著上调，表明其在增强耐盐性方面的潜在作用。相反，3和4表现出下调，表明在响应不同盐胁迫水平时存在复杂的调控机制。这些发现将增进我们对NCED基因家族遗传组成和进化发展的理解，并为未来研究它们在响应盐诱导胁迫中的作用提供基础。
