Zhang Yong, Han Peng, Zhao Ruijie, Yu Shuhan, Liu Hang, Wu Hong, Weng Jinyang, Zhang Hengfeng
School of Landscape Architecture and Horticulture, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China.
Plants (Basel). 2024 Nov 29;13(23):3357. doi: 10.3390/plants13233357.
Blue fescue is a widely used ornamental grass because of its strong ecological adaptability. To maintain the optimal ornamental plant shape, blue fescue requires many nutrients and labor. Using dwarf varieties with slow growth is an effective way to fulfill these requirements. In this study, we investigated the dwarfing mechanism of , a blue fescue dwarfing mutant, using physiological, transcriptomic, and proteomic methods. The peroxidase (POD) enzyme activity and chlorophyll content of significantly increased, while the lignin, gibberellin (GA), and indoleacetic acid (IAA) content significantly decreased. A total of 7668 differentially expressed genes (DEGs) were detected using RNA-seq, of which 2543 were upregulated and 5125 were downregulated. A total of 165 differentially expressed proteins (DEPs) were detected using iTRAQ, of which 68 were upregulated and 97 were downregulated. KEGG enrichment analysis showed that the diterpene biosynthesis pathway, tryptophan metabolism pathway, and phenylpropanoid biosynthesis pathway were significantly enriched at both the transcriptional and protein levels. As a result, we can formulate the following hypothesis about the dwarfing phenotype: the downregulation of genes and proteins related to IAA and GA biosynthesis is associated with the dwarf phenotype's formation, and metabolic pathways related to lignin synthesis, such as phenylpropanoid biosynthesis, also play an important role. Our work will contribute to a new understanding of the genes and proteins involved in the blue fescue dwarf phenotype.
蓝羊茅因其较强的生态适应性而被广泛用作观赏草。为保持最佳观赏植物形态，蓝羊茅需要大量养分和人工。使用生长缓慢的矮化品种是满足这些需求的有效途径。在本研究中，我们采用生理、转录组和蛋白质组学方法，研究了蓝羊茅矮化突变体的矮化机制。其过氧化物酶（POD）活性和叶绿素含量显著增加，而木质素、赤霉素（GA）和吲哚乙酸（IAA）含量显著降低。通过RNA-seq共检测到7668个差异表达基因（DEG），其中2543个上调，5125个下调。通过iTRAQ共检测到165个差异表达蛋白（DEP），其中68个上调，97个下调。KEGG富集分析表明，二萜生物合成途径、色氨酸代谢途径和苯丙烷生物合成途径在转录和蛋白质水平均显著富集。因此，我们可以对该蓝羊茅矮化表型提出以下假设：与IAA和GA生物合成相关的基因和蛋白质的下调与矮化表型的形成有关，与木质素合成相关的代谢途径，如苯丙烷生物合成，也起着重要作用。我们的工作将有助于对参与蓝羊茅矮化表型的基因和蛋白质有新的认识。
