Luo Xiaomei, Liu Yunke, Lei Yuting, He Zhoujian, Gong Xiao, Ye Meng, Xiao Qiangang
National Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, College of Forestry, Sichuan Agricultural University, Chengdu 611130, China.
Chengdu Academy of Agriculture and Forestry Sciences, Nongke Road 200, Wenjiang District, Chengdu 611130, China.
Plants (Basel). 2024 Dec 3;13(23):3394. doi: 10.3390/plants13233394.
from Sichuan is a valuable germplasm with high economic potential, but it faces variety scarcity. To address this, this study collected 16 varieties (lines), identifying IpHT1 as a promising parent due to its high oil content (38.5%) and red fruits. Polyploid induction via adding 0.50% colchicine to Murashige and Skoog (MS) medium yielded 520 IpHT1 mutagenized seedlings. Subsequently, flow cytometry (FCM) was performed on 401 morphologically variant seedlings which had been initially screened, resulting in the identification of 15 suspected triploids, 35 suspected tetraploids, and 3 chimeras. Furthermore, fluorescence in situ hybridization (FISH) analysis found that the probe (AGT) had terminal signals at both ends of each chromosome, allowing for the counting of 42 chromosomes in diploids and 84 in tetraploids. The probe 5S rDNA showed 2, 3, and 4 hybridization signals in the interphase nuclei of diploid, triploid, and tetraploid cells, respectively, but the probe (GAA) failed to produce any signal on chromosomes. Ultimately, 18 polyploids were selected, including 7 triploids and 11 tetraploids. Triploids and tetraploids showed significant leaf morphological and physiological differences from diploids. Consequently, this study successfully established a polyploid breeding system for , thereby enhancing its genetic diversity and breeding potential.
来自四川的[品种名称未提及]是一种具有高经济潜力的宝贵种质资源，但面临品种稀缺的问题。为解决这一问题，本研究收集了16个品种（品系），由于其高含油量（38.5%）和红色果实，将IpHT1鉴定为一个有前景的亲本。通过向Murashige和Skoog（MS）培养基中添加0.50%秋水仙素进行多倍体诱导，获得了520株IpHT1诱变幼苗。随后，对最初筛选出的401株形态变异幼苗进行了流式细胞术（FCM）分析，鉴定出15株疑似三倍体、35株疑似四倍体和3株嵌合体。此外，荧光原位杂交（FISH）分析发现，探针（AGT）在每条染色体的两端都有末端信号，二倍体中可计数42条染色体，四倍体中可计数84条染色体。探针5S rDNA在二倍体、三倍体和四倍体细胞的间期核中分别显示2、3和4个杂交信号，但探针（GAA）在染色体上未产生任何信号。最终，选择了18个多倍体，包括7个三倍体和11个四倍体。三倍体和四倍体在叶片形态和生理上与二倍体表现出显著差异。因此，本研究成功建立了[品种名称未提及]的多倍体育种体系，从而提高了其遗传多样性和育种潜力。
