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bHLH转录因子PhbHLH121调控对缺铁的响应 。 （原文句末不完整，推测是在某种植物等对象中，这里按字面翻译）

The bHLH Transcription Factor PhbHLH121 Regulates Response to Iron Deficiency in .

作者信息

Pan Liru, Huang Chengcheng, Li Ruiling, Li Yanbang

机构信息

School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

出版信息

Plants (Basel). 2024 Dec 6;13(23):3429. doi: 10.3390/plants13233429.

DOI:10.3390/plants13233429
PMID:39683222
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11644503/
Abstract

Iron (Fe) is an essential micronutrient for plants. Due to the low Fe bioavailability in cultivated soils, Fe deficiency is a widespread agricultural problem. In this study, we present the functional characterization of a petunia () basic-helix-loop-helix transcription factor PhbHLH121 in response to Fe shortage. Real-time PCR revealed that the expression of in petunia roots and shoots was downregulated under Fe-limited conditions. CRISPR/Cas9-edited mutant plants were generated to investigate the functions of PhbHLH121 in petunia. Loss-of-function of PhbHLH121 enhanced petunia tolerance to Fe deficiency. Further investigations revealed that the expression level of several structural genes involved in Fe uptake in petunia, such as and , was higher in mutants compared to that in wild-type under Fe-limited conditions, and the expression level of several genes involved in Fe storage and Fe transport, such as , and , was lower in mutants compared to that in wild-type under Fe-deficient conditions. Yeast one-hybrid assays revealed that PhbHLH121 binds to the G-box element in the promoter of genes involved in Fe homeostasis. Yeast two-hybrid assays revealed that PhbHLH121 interacts with petunia bHLH IVc proteins. Taken together, PhbHLH121 plays an important role in the Fe deficiency response in petunia.

摘要

铁（Fe）是植物必需的微量营养元素。由于耕作土壤中铁的生物有效性较低，缺铁是一个普遍存在的农业问题。在本研究中，我们展示了矮牵牛（）碱性螺旋-环-螺旋转录因子PhbHLH121对铁短缺的功能特性。实时定量PCR显示，在铁限制条件下，矮牵牛根和茎中该基因的表达下调。我们通过CRISPR/Cas9编辑产生了该基因的突变植株，以研究PhbHLH121在矮牵牛中的功能。PhbHLH121功能缺失增强了矮牵牛对缺铁的耐受性。进一步研究表明，在铁限制条件下，矮牵牛中几个参与铁吸收的结构基因，如和，在突变体中的表达水平高于野生型，而在缺铁条件下，几个参与铁储存和铁运输的基因，如、和，在突变体中的表达水平低于野生型。酵母单杂交试验表明，PhbHLH121与参与铁稳态的基因启动子中的G-box元件结合。酵母双杂交试验表明，PhbHLH121与矮牵牛bHLH IVc蛋白相互作用。综上所述，PhbHLH121在矮牵牛缺铁反应中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/f4808d85163c/plants-13-03429-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/7596de763f69/plants-13-03429-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/147b847a9191/plants-13-03429-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/c58e8453cf33/plants-13-03429-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/534c1ba272fb/plants-13-03429-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/37d852673991/plants-13-03429-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/f8893b0f23ce/plants-13-03429-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/c0942cad5743/plants-13-03429-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/f4808d85163c/plants-13-03429-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/7596de763f69/plants-13-03429-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/147b847a9191/plants-13-03429-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/c58e8453cf33/plants-13-03429-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/534c1ba272fb/plants-13-03429-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/37d852673991/plants-13-03429-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/f8893b0f23ce/plants-13-03429-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/c0942cad5743/plants-13-03429-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/061b/11644503/f4808d85163c/plants-13-03429-g008.jpg

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MsMYB741 is involved in alfalfa resistance to aluminum stress by regulating flavonoid biosynthesis.MsMYB741 通过调控类黄酮生物合成参与苜蓿耐铝胁迫。
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Iron uptake, signaling, and sensing in plants.植物中铁的摄取、信号转导和感应。
Plant Commun. 2022 Sep 12;3(5):100349. doi: 10.1016/j.xplc.2022.100349. Epub 2022 Jun 14.
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bHLH11 inhibits bHLH IVc proteins by recruiting the TOPLESS/TOPLESS-RELATED corepressors.bHLH11 通过招募 TOPLESS/TOPLESS 相关的核心抑制蛋白来抑制 bHLH IVc 蛋白。
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Transcriptome Profiling of Cu Stressed Petunia Petals Reveals Candidate Genes Involved in Fe and Cu Crosstalk.铜胁迫矮牵牛花瓣转录组分析揭示了参与铁铜交叉对话的候选基因。
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Identification of Plant Growth Promoting Rhizobacteria That Improve the Performance of Greenhouse-Grown Petunias under Low Fertility Conditions.在低肥力条件下提高温室种植矮牵牛生长性能的植物促生根际细菌的鉴定
Plants (Basel). 2021 Jul 9;10(7):1410. doi: 10.3390/plants10071410.
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