Ben Kaab Sofiene, Fernández Pierna Juan Antonio, Foncoux Berenice, Compère Philippe, Baeten Vincent, Jijakli M Haïssam
Integrated and Urban Plant Pathology Laboratory, University of Liège, Gembloux Agro-Bio Tech, 2 Passage des Déportés, 5030 Gembloux, Belgium.
Quality and Authentication of Products Unit, Knowledge and Valorization of Agricultural Products Department, Walloon Agricultural Research Centre (CRA-W), Chaussée de Namur 24, 5030 Gembloux, Belgium.
Plants (Basel). 2024 Dec 6;13(23):3432. doi: 10.3390/plants13233432.
The use of chemical herbicides induces negative impacts on the environment, animals, and human health. It also leads to the development of herbicide-resistant weeds. In this context, natural and efficacious herbicides are highly sought after. Essential oils are natural compounds with antibacterial, fungicidal, and phytotoxic properties. For this reason, we studied the post-emergence phytotoxic effect of cinnamon essential oil (cinnamon EO) from under greenhouse conditions, testing it against () and (). The content of malondialdehyde (MDA), percentage of water loss, electrolyte leakage, and the fluorescence of treated leaves by cinnamon EO were determined in order to understand the physiological and biochemical responses. In addition, transmission electron microscopy (TEM) was used to study the effect of cinnamon EO on cellular organelles in different tissues of leaves. Results showed that cinnamon EO quickly induced oxidative stress in treated leaves by increasing MDA content, impacting membrane integrity and causing water loss. TEM observations confirmed the cell desiccation by cellular plasmolysis and showed an alteration of the membrane integrity and chloroplast damages. Moreover, Raman analysis confirms the disturbance of the plant metabolism by the disappearance of some scattering bands which correspond to primary metabolites. Through our finding, we confirm that cinnamon essential oil (EO) could be proposed in the future as a potential bioherbicide and a suitable source of natural phytotoxic compounds with a multisite action on weeds.
化学除草剂的使用会对环境、动物和人类健康产生负面影响。它还会导致抗除草剂杂草的出现。在此背景下，天然且有效的除草剂备受青睐。精油是具有抗菌、杀菌和植物毒性特性的天然化合物。因此，我们在温室条件下研究了肉桂精油（肉桂EO）对杂草的苗后植物毒性作用，并对其进行了测试。测定了丙二醛（MDA）含量、水分流失百分比、电解质渗漏以及肉桂EO处理叶片的荧光，以了解其生理和生化反应。此外，还利用透射电子显微镜（TEM）研究了肉桂EO对杂草叶片不同组织细胞器的影响。结果表明，肉桂EO通过增加MDA含量，影响膜完整性并导致水分流失，从而迅速在处理过的叶片中诱导氧化应激。TEM观察证实了细胞因质壁分离而干燥，并显示出膜完整性改变和叶绿体损伤。此外，拉曼分析通过一些对应于初级代谢物的散射带的消失证实了植物代谢的紊乱。通过我们的研究发现，我们证实肉桂精油（EO）未来可作为一种潜在的生物除草剂以及对杂草具有多位点作用的天然植物毒性化合物的合适来源。
