Filippova Arina D, Baranchikov Alexander E, Teplonogova Maria A, Savintseva Irina V, Popov Anton L, Ivanov Vladimir K
Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prospect, 31, Moscow 119991, Russia.
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya Str., 3, Pushchino 142290, Russia.
Nanomaterials (Basel). 2024 Nov 27;14(23):1908. doi: 10.3390/nano14231908.
Cerium dioxide sols stabilised with L-malic acid were shown to exhibit significant antioxidant activity towards alkyl peroxyl radicals in the range of ligand:CeO molar ratios of 0.2-1 (0.2:1, 0.4:1, 0.5:1, 0.6:1, 0.8:1 and 1:1). The antioxidant activity of cerium dioxide nanoparticles greatly depended on L-malic acid content and increased by 8 times when the ligand:CeO molar ratio increased from 0.2:1 to 0.4:1. An estimate of the ligand:CeO molar ratio required to ensure complete surface coverage of CeO nanoparticles with malate anions resulted in a value of 0.2. Aggregation degree of CeO nanoparticles depends on the ligand:CeO molar ratio. In the range of ligand:CeO molar ratios 0.2-0.4, the size of aggregates decreased by an order of magnitude. The antioxidant capacity of 1 mM malate-stabilised cerium dioxide (0.2:1) relative to sodium ascorbate was 0.012 ± 0.001 mM. The antioxidant activity of cerium dioxide stabilised with L-malic acid at a ligand:CeO molar ratio of 0.2:1 was 80 times less than the antioxidant activity of sodium ascorbate. Cerium dioxide nanoparticles stabilised with L-malic acid did not demonstrate a cytotoxic effect against human mesenchymal stem cells, in a wide range of concentrations (10-10 M), and their proliferation was stimulated after 72 h of cultivation. The results obtained show new possibilities for the design of biocompatible ceria-based nanomaterials with tunable pro- and antioxidant properties; these materials can further be assessed in view of their potential for treating oxidative stress-related disorders.
结果表明，在配体与二氧化铈的摩尔比为0.2 - 1（0.2:1、0.4:1、0.5:1、0.6:1、0.8:1和1:1）范围内，用L - 苹果酸稳定的二氧化铈溶胶对烷基过氧自由基表现出显著的抗氧化活性。二氧化铈纳米颗粒的抗氧化活性在很大程度上取决于L - 苹果酸的含量，当配体与二氧化铈的摩尔比从0.2:1增加到0.4:1时，抗氧化活性增加了8倍。经估算，要确保二氧化铈纳米颗粒表面完全被苹果酸根阴离子覆盖，所需的配体与二氧化铈的摩尔比为0.2。二氧化铈纳米颗粒的聚集程度取决于配体与二氧化铈的摩尔比。在配体与二氧化铈的摩尔比为0.2 - 0.4范围内，聚集体的尺寸减小了一个数量级。相对于抗坏血酸钠，1 mM苹果酸稳定的二氧化铈（0.2:1）的抗氧化能力为0.012±0.001 mM。在配体与二氧化铈的摩尔比为0.2:1时，用L - 苹果酸稳定的二氧化铈的抗氧化活性比抗坏血酸钠的抗氧化活性低80倍。用L - 苹果酸稳定的二氧化铈纳米颗粒在广泛的浓度范围（10⁻¹⁰ - 10⁻⁶ M）内对人间充质干细胞未表现出细胞毒性作用，并且在培养72小时后其增殖受到刺激。所得结果为设计具有可调促氧化和抗氧化特性的生物相容性二氧化铈基纳米材料展示了新的可能性；鉴于这些材料在治疗氧化应激相关疾病方面的潜力，可对其进行进一步评估。
