D'Elia Maria, Marino Carmen, Celano Rita, Napolitano Enza, D'Ursi Anna Maria, Russo Mariateresa, Rastrelli Luca
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano, Italy.
National Biodiversity Future Center-NBFC, 90133 Palermo, Italy.
Nutrients. 2024 Nov 28;16(23):4096. doi: 10.3390/nu16234096.
: This study investigates the effectiveness of an herbal formulation, STRESSLESS (ST-65), which combines ashwagandha () and bacopa (), on SH-SY5Y human neuroblastoma cells. Given the rising interest in natural compounds for neuroprotection and stress alleviation, we aimed to explore the cellular and molecular effects of this formulation. : Utilizing a nuclear magnetic resonance (NMR) metabolomic approach and ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS), we identified key bioactive compounds in ST-65, including withanolides from ashwagandha and bacosides from bacopa. : Our findings indicate that ST-65 treatment significantly alters the metabolic profile of SH-SY5Y cells. Key changes included increased levels of metabolites linked to neuroprotection, energy metabolism, and antioxidant defense. Notable enhancements were observed in specific amino acids and neuroprotective compounds, suggesting activation of neuroprotective mechanisms and mitigation of stress-induced damage. : The study reveals a complex phyto-chemical profile of ST-65 and underscores its potential as a natural active agent for addressing stress-related neurodegenerative conditions. These insights into neuronal mechanisms provide a foundation for further exploration of herbal formulations in neuroprotection.
本研究调查了一种草药配方STRESSLESS（ST - 65）的有效性，该配方将南非醉茄和假马齿苋相结合，作用于SH - SY5Y人神经母细胞瘤细胞。鉴于人们对用于神经保护和减轻压力的天然化合物的兴趣日益浓厚，我们旨在探索该配方的细胞和分子效应。：利用核磁共振（NMR）代谢组学方法和超高效液相色谱 - 高分辨率质谱（UHPLC - HRMS），我们鉴定了ST - 65中的关键生物活性化合物，包括来自南非醉茄的茄内酯和来自假马齿苋的假马齿苋皂甙。：我们的研究结果表明，ST - 65处理显著改变了SH - SY5Y细胞的代谢谱。关键变化包括与神经保护、能量代谢和抗氧化防御相关的代谢物水平增加。在特定氨基酸和神经保护化合物中观察到显著增强，表明神经保护机制被激活，应激诱导的损伤得到缓解。：该研究揭示了ST - 65复杂的植物化学特征，并强调了其作为解决与压力相关的神经退行性疾病的天然活性剂的潜力。这些对神经元机制的见解为进一步探索草药配方在神经保护中的应用提供了基础。
