靛玉红通过靶向粒细胞-巨噬细胞集落刺激因子介导的PI3K-AKT-NF-κB轴下调制瘤素M

Tryptanthrin Down-Regulates Oncostatin M by Targeting GM-CSF-Mediated PI3K-AKT-NF-κB Axis.

作者信息

Han Na-Ra, Park Hi-Joon, Ko Seong-Gyu, Moon Phil-Dong

机构信息

College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.

Korean Medicine-Based Drug Repositioning Cancer Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.

出版信息

Nutrients. 2024 Nov 28;16(23):4109. doi: 10.3390/nu16234109.

BACKGROUND

Oncostatin M (OSM) is involved in several inflammatory responses. Tryptanthrin (TRYP), as a natural alkaloid, is a bioactive compound derived from indigo plants. Objectives/ Methods: The purpose of this study is to investigate the potential inhibitory activity of TRYP on OSM release from neutrophils using neutrophils-like differentiated (d)HL-60 cells and neutrophils from mouse bone marrow.

RESULTS

The results showed that TRYP reduced the production and mRNA expression levels of OSM in the granulocyte-macrophage colony-stimulating factor (GM-CSF)-stimulated neutrophils-like dHL-60 cells. In addition, TRYP decreased the OSM production levels in the GM-CSF-stimulated neutrophils from mouse bone marrow. TRYP inhibited the phosphorylation of phosphatidylinositol 3-kinase (PI3K), AKT, and nuclear factor (NF)-κB in the GM-CSF-stimulated neutrophils-like dHL-60 cells.

CONCLUSIONS

Therefore, these results reveal for the first time that TRYP inhibits OSM release via the down-regulation of PI3K-AKT-NF-κB axis from neutrophils, presenting its potential as a therapeutic agent for inflammatory responses.

背景

抑瘤素M（OSM）参与多种炎症反应。色胺酮（TRYP）作为一种天然生物碱，是一种从靛蓝植物中提取的生物活性化合物。目的/方法：本研究旨在利用中性粒细胞样分化的（d）HL-60细胞和小鼠骨髓来源的中性粒细胞，研究TRYP对中性粒细胞释放OSM的潜在抑制活性。

结果

结果显示，TRYP降低了粒细胞-巨噬细胞集落刺激因子（GM-CSF）刺激的中性粒细胞样dHL-60细胞中OSM的产生和mRNA表达水平。此外，TRYP降低了GM-CSF刺激的小鼠骨髓中性粒细胞中OSM的产生水平。TRYP抑制了GM-CSF刺激的中性粒细胞样dHL-60细胞中磷脂酰肌醇3激酶（PI3K）、AKT和核因子（NF）-κB的磷酸化。