Kim Hyeon-Ji, Mun Ji-Su, Oh Suk-Heung, Kim Jun-Hyung
Department of Food and Biotechnology, Woosuk University, Wanju 55338, Jeonbuk-do, Republic of Korea.
BIOMAYSIN, Jeongeup 56212, Jeonbuk-do, Republic of Korea.
Nutrients. 2024 Nov 30;16(23):4168. doi: 10.3390/nu16234168.
BACKGROUND/OBJECTIVES: In aerobic organisms, such as humans, oxygen radicals are inevitably produced. To counteract oxidation, the body generates antioxidant substances that suppress free radicals. However, levels of reactive oxygen species (ROS) increase due to aging and lifestyle factors, leading to exposure to various diseases. While synthetic antioxidants offer advantages like high stability, low cost, and availability, their safety remains controversial. This study aimed to investigate the antioxidant and antiaging activities of (HC), which is rich in flavonoids and has excellent antioxidant properties, using as a model.
Extraction and fractionation of HC were performed to evaluate antioxidant activities (DPPH, ABTS, superoxide radical scavenging activity) and antiaging effects (lifespan). The ethyl acetate fraction (EAF) with the highest activity was selected for further investigation.
The EAF of HC exhibited high levels of polyphenols and flavonoids, presenting the highest DPPH, ABTS, and superoxide radical scavenging activities. This fraction increased the activity of antioxidant enzymes in nematodes in a concentration-dependent manner and provided resistance to oxidative stress, reducing ROS accumulation. Additionally, the fraction enhanced the lifespan of nematodes, improved resistance to heat stress, increased survival rates, and decreased the accumulation of aging pigments (lipofuscin). The expression of daf-2, daf-16, and sir-2.1, proteins directly involved in nematode aging, was confirmed. Liquid chromatography/tandem mass spectrometry identified quercitrin in the HC extract, which may contribute to its antioxidant and antiaging effects.
The EAF of HC demonstrates significant potential for influencing antioxidant and antiaging, as evidenced by functional investigations using .
背景/目的：在需氧生物（如人类）中，氧自由基不可避免地产生。为了对抗氧化，身体会产生抑制自由基的抗氧化物质。然而，由于衰老和生活方式因素，活性氧（ROS）水平会升高，导致易患各种疾病。虽然合成抗氧化剂具有高稳定性、低成本和易获得等优点，但其安全性仍存在争议。本研究旨在以线虫为模型，研究富含黄酮类化合物且具有优异抗氧化性能的[具体物质名称未给出，暂用“（HC）”表示]的抗氧化和抗衰老活性。
对HC进行提取和分离，以评估其抗氧化活性（DPPH、ABTS、超氧阴离子自由基清除活性）和抗衰老作用（寿命）。选择活性最高的乙酸乙酯馏分（EAF）进行进一步研究。
HC的EAF表现出高水平的多酚和黄酮类化合物，具有最高的DPPH、ABTS和超氧阴离子自由基清除活性。该馏分以浓度依赖的方式增加线虫体内抗氧化酶的活性，并提供对氧化应激的抗性，减少ROS积累。此外，该馏分延长了线虫的寿命，提高了对热应激的抗性，增加了存活率，并减少了衰老色素（脂褐素）的积累。证实了直接参与线虫衰老的蛋白质daf - 2、daf - 16和sir - 2.1的表达。液相色谱/串联质谱法鉴定出HC提取物中的槲皮苷，这可能有助于其抗氧化和抗衰老作用。
HC的EAF在影响抗氧化和抗衰老方面显示出巨大潜力，这通过使用线虫进行的功能研究得到了证明。
