Kim Hyeon-Ji, Mun Ji-Su, Oh Suk-Heung, Kim Jun-Hyeong
Department of Food and Biotechnology, Woosuk University, Jeonbuk 55338, Korea.
Prev Nutr Food Sci. 2024 Sep 30;29(3):311-320. doi: 10.3746/pnf.2024.29.3.311.
Reactive oxygen species (ROS) are produced from energy metabolism and may cause diseases or cell death. Antioxidation refers to the suppression of ROS production and is considered beneficial in preventing diseases. This study aimed to examine the antioxidative effects of Makino (COM) extracts and fractions using as an experimental model. The COM ethanol extract was fractionated according to polarity. The results showed that the ethyl acetate fraction of COM showed powerful radical scavenging activities and increased the activities of superoxide dismutase (SOD) and catalase in in a concentration-dependent manner. Moreover, the ethyl acetate fraction reduced the ROS production rate in and increased the cell survival rate, suggesting oxidative and thermal stress resistance. In addition, the SOD-3::green fluorescent protein (GFP) expression level in the transformed cells of (CF1553) increased, suggesting oxidative stress resistance. Similarly, the HSP-16.2::GFP expression level increased, suggesting thermal stress resistance. In conclusion, the ethyl acetate fraction of COM demonstrated the strongest antioxidative effects, indicating that it may help extend longevity.
活性氧（ROS）由能量代谢产生，可能导致疾病或细胞死亡。抗氧化作用是指抑制ROS的产生，被认为对预防疾病有益。本研究旨在以秀丽隐杆线虫作为实验模型，检测牧野氏线虫（COM）提取物及其组分的抗氧化作用。COM乙醇提取物根据极性进行分离。结果显示，COM的乙酸乙酯组分表现出强大的自由基清除活性，并以浓度依赖的方式提高了秀丽隐杆线虫中超氧化物歧化酶（SOD）和过氧化氢酶的活性。此外，乙酸乙酯组分降低了秀丽隐杆线虫中的ROS产生率，并提高了细胞存活率，表明其具有抗氧化和抗热应激能力。另外，秀丽隐杆线虫（CF1553）转化细胞中的SOD-3::绿色荧光蛋白（GFP）表达水平升高，表明其具有抗氧化应激能力。同样，HSP-16.2::GFP表达水平升高，表明其具有抗热应激能力。总之，COM的乙酸乙酯组分表现出最强的抗氧化作用，表明它可能有助于延长寿命。
