Haro-Reyes Jose, Raghupathi Jayaprakash Kanijam, Reddivari Lavanya
Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.
Department of Chemistry, Acharya Nagarjuna University, Guntur 522510, Andhra Pradesh, India.
Nutrients. 2024 Dec 7;16(23):4232. doi: 10.3390/nu16234232.
Gut bacterial dysbiosis along with intestinal mucosal disruption plays a critical role in inflammatory disorders like ulcerative colitis. Flavonoids and other food bioactives have been studied in mice models as alternative treatments with minimal side effects. However, most of the research has been carried out with mice-native microbiota, which limits the comprehension of the interaction between flavonoids and human-associated bacteria. Hence, the objective of our study was to determine the effect of healthy human-associated microbiota on the anti-colitic activity of diets rich in anthocyanins (3-HF) and phlobaphenes (3-DF).
In this regard, the interleukin (IL)-10 -/- mice model was utilized. Mice were divided into three groups for inoculation with human gut bacteria from three different healthy donors and assigned to four diets. A purified diet (Diet P) and three diets containing 25% near-isogenic lines (NILs) of corn were evaluated. Diets were substituted with NILs expressing only 3-DFs (diet B), only 3-HFs (diet C), and both 3-DF and 3-HF (diet D).
In an overall analysis, flavonoid-rich diets did not affect inflammatory markers, microbiota diversity, or gut metabolites, but diets containing anthocyanins improved barrier function parameters. However, when data was segmented by the recipient's microbiota from different human donors, the diet effects became significant. Furthermore, 3-HFs showed more beneficial effects than 3-DFs across the recipient's microbiota.
Our study suggests that the anti-colitic activity of 3-DF and 3-HF and their gut metabolites depends on the donor's microbial composition.
肠道细菌生态失调以及肠黏膜破坏在溃疡性结肠炎等炎症性疾病中起关键作用。黄酮类化合物和其他食物生物活性物质已在小鼠模型中作为副作用最小的替代疗法进行了研究。然而，大多数研究是针对小鼠原生微生物群进行的，这限制了对黄酮类化合物与人类相关细菌之间相互作用的理解。因此，我们研究的目的是确定健康的人类相关微生物群对富含花青素（3-HF）和鞣红（3-DF）的饮食的抗结肠炎活性的影响。
在这方面，使用了白细胞介素（IL）-10基因敲除小鼠模型。将小鼠分为三组，接种来自三名不同健康供体的人类肠道细菌，并分配到四种饮食中。评估了一种纯化饮食（饮食P）和三种含有25%玉米近等基因系（NILs）的饮食。饮食被分别用仅表达3-DF的NILs（饮食B）、仅表达3-HF的NILs（饮食C）以及同时表达3-DF和3-HF的NILs（饮食D）替代。
在总体分析中，富含黄酮类化合物的饮食不影响炎症标志物、微生物群多样性或肠道代谢产物，但含有花青素的饮食改善了屏障功能参数。然而，当按不同人类供体的受体微生物群对数据进行细分时，饮食效果变得显著。此外，在受体微生物群中，3-HF比3-DF显示出更有益的效果。
我们的研究表明，3-DF和3-HF及其肠道代谢产物的抗结肠炎活性取决于供体的微生物组成。
