Wu Haizhi, Si Shihui, Li Zheng, Su Jiayou, Jia Shangguan, He Hao, Peng Chengcheng, Cheng Tongqiang, Wu Qian
Hunan Provincial Institute of Product and Goods Quality Inspection, Changsha 410007, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Molecules. 2024 Dec 2;29(23):5699. doi: 10.3390/molecules29235699.
In this study, an aptamer biosensor for detecting lactoferrin (LF) was developed using piezoelectric quartz-induced bond rupture sensing technology. The thiol-modified aptamer I was immobilized on the gold electrode surface of the quartz crystal microbalance (QCM) through an Au-S bond to specifically bind LF. It was then combined with aptamer-magnetic beads to amplify the mass signal. The peak excitation voltage was 8 V at the resonance frequency for the 60 MHz gold-plated quartz crystal. When the molecular bond cracking process occurred, the aptamer-magnetic beads combined on the surface of the piezoelectric quartz were removed, which resulted in an increase in quartz crystal resonance frequency. Therefore, the specific detection of LF can be realized. Under optimized experimental conditions, the linear range for LF was 10-500 ng/mL, the detection limit (3σ) was 8.2 ng/mL, and the sample recoveries for actual milk powder samples ranged from 97.2% to 106.0%. Compared with conventional QCM sensing technology, the signal acquisition process of this sensing method is simple, fast, and easy to operate.
在本研究中，利用压电石英诱导键断裂传感技术开发了一种用于检测乳铁蛋白（LF）的适体生物传感器。巯基修饰的适体I通过Au-S键固定在石英晶体微天平（QCM）的金电极表面，以特异性结合LF。然后将其与适体磁珠结合以放大质量信号。对于60 MHz镀金石英晶体，在共振频率下峰值激励电压为8 V。当发生分子键断裂过程时，结合在压电石英表面的适体磁珠被去除，这导致石英晶体共振频率增加。因此，可以实现LF的特异性检测。在优化的实验条件下，LF的线性范围为10 - 500 ng/mL，检测限（3σ）为8.2 ng/mL，实际奶粉样品的加标回收率为97.2%至106.0%。与传统的QCM传感技术相比，这种传感方法的信号采集过程简单、快速且易于操作。
