Valina Anna A, Siniukova Vera A, Belashova Tatyana A, Kanapin Alexander A, Samsonova Anastasia A, Masharsky Alexey E, Lykholay Anna N, Galkina Svetlana A, Zadorsky Sergey P, Galkin Alexey P
St. Petersburg Branch, Vavilov Institute of General Genetics, Russian Academy of Sciences, Universitetskaya Emb. 7/9, 199034 St. Petersburg, Russia.
Department of Genetics and Biotechnology, Faculty of Biology, St. Petersburg State University, Universitetskaya Emb. 7/9, 199034 St. Petersburg, Russia.
Int J Mol Sci. 2024 Nov 21;25(23):12499. doi: 10.3390/ijms252312499.
is the oldest classic model object in developmental genetics. It may seem that various structures of the fruit fly at all developmental stages have been well studied and described. However, recently we have shown that some specialized structures of the eggshell contain an amyloid fibril network. Here, we demonstrate that this amyloid network is formed by the chorionic protein s36. The s36 protein colocalizes with the amyloid-specific dyes Congo Red and Thioflavin S in the micropyle, dorsal appendages, and pillars. The fibrils of s36 obtained from the eggs demonstrate amyloid properties. In the context of the gene deletion, the s36 protein is produced but is not detected in the eggshell. The absence of amyloid fibrils of s36 in the eggshell disrupts the endochorion morphology and blocks the development of the micropyle, dorsal appendages, and pillars, leading to sterility. Our data show for the first time that amyloid fibrils are essential for morphogenesis modulation. We suggest that attachment of follicle cells to the s36 extracellular fibrils triggers signaling to enable subsequent cellular divisions needed for building the specialized eggshell structures.
是发育遗传学中最古老的经典模型对象。果蝇在所有发育阶段的各种结构似乎都已得到充分研究和描述。然而，最近我们发现果蝇卵壳的一些特殊结构含有淀粉样纤维网络。在此，我们证明这种淀粉样网络是由绒毛膜蛋白s36形成的。s36蛋白在卵孔、背附肢和支柱中与淀粉样特异性染料刚果红和硫黄素S共定位。从卵中获得的s36纤维具有淀粉样特性。在基因缺失的情况下，s36蛋白产生但在卵壳中未检测到。卵壳中s36淀粉样纤维的缺失会破坏内膜形态并阻碍卵孔、背附肢和支柱的发育，导致不育。我们的数据首次表明淀粉样纤维对形态发生调节至关重要。我们认为卵泡细胞与s36细胞外纤维的附着触发信号传导，以实现构建特殊卵壳结构所需的后续细胞分裂。
