González-Cardona Carolina, López Walter Ricardo, Jovel Juan, Soto-Suárez Mauricio, Ceballos-Aguirre Nelson
Facultad de Ciencias Agropecuarias, Universidad de Caldas, Calle 65 No. 26-10, Manizales 170003, Caldas, Colombia.
Departamento de Física y Química, Facultad de Ciencias Naturales, Universidad Nacional de Colombia Sede Manizales, km 9 vía Aeropuerto la Nubia, Manizales 170003, Caldas, Colombia.
Int J Mol Sci. 2024 Nov 23;25(23):12584. doi: 10.3390/ijms252312584.
Meloidogyne causes a devastating disease known as root-knot that affects tomatoes and other cash crops worldwide. Conversely, has proven beneficial in mitigating the effects of various pathogens in plants. We aimed to unravel the molecular events that underlie the beneficial effects of the bacterium and the detrimental impacts of the nematode when inoculated separately or together in tomato plants. The transcriptional responses induced by (TB group (tomato-bacteria group)), spp. (TN group (tomato-nematode group)) or by the two agents (TBN group (tomato-bacteria-nematode group)) in tomato were assessed by RNA-seq. We implemented a transcript discovery pipeline which allowed the identification of 2283 putative novel transcripts. Differential expression analysis revealed that upregulated transcripts were much more numerous than downregulated ones. At the gene ontology level, the most activated term was 'hydrolase activity acting on ester bonds' in all groups. In addition, when both microbes were inoculated together, 'hydrolase activity acting on O-glycosyl compounds' was activated. This finding suggests defense responses related to lipid and carbohydrate metabolism, membrane remodeling and signal transduction. Notably, defense genes, transcription factors and protein kinases stood out. Differentially expressed transcripts suggest the activation of a multifaceted plant defense response against the nematode occurred, which was exacerbated by pre-inoculation of .
根结线虫会引发一种名为根结病的毁灭性病害，影响全球范围内的番茄和其他经济作物。相反，[此处原文缺失相关细菌名称]已被证明在减轻植物中各种病原体的影响方面具有益处。我们旨在揭示当该细菌和线虫分别或共同接种到番茄植株中时，其产生有益效果和有害影响背后的分子事件。通过RNA测序评估了[此处原文缺失相关细菌名称]（TB组（番茄-细菌组））、根结线虫属（TN组（番茄-线虫组））或这两种病原体（TBN组（番茄-细菌-线虫组））在番茄中诱导的转录反应。我们实施了一个转录本发现流程，从而能够鉴定出2283个推定的新转录本。差异表达分析表明，上调的转录本比下调的转录本数量多得多。在基因本体水平上，所有组中最活跃的术语是“作用于酯键的水解酶活性”。此外，当两种微生物一起接种时，“作用于O-糖基化合物的水解酶活性”被激活。这一发现表明了与脂质和碳水化合物代谢、膜重塑及信号转导相关的防御反应。值得注意的是，防御基因、转录因子和蛋白激酶尤为突出。差异表达的转录本表明，植物针对线虫发生了多方面防御反应的激活，而[此处原文缺失相关细菌名称]的预先接种加剧了这种反应。
