Tang Gangliang, Li Xiangyi, Zeng Fanjiang, Ma Junning, Guan Pingyin, Zhang Bo
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Xinjiang Key Laboratory of Desert Plant Roots Ecology and Vegetation Restoration, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Int J Mol Sci. 2024 Nov 27;25(23):12725. doi: 10.3390/ijms252312725.
, a desert shrub known for its impressive drought tolerance, exhibits notable resilience under arid conditions. However, the underlying mechanisms driving its drought resistance remain largely unexplored. This study aims to investigate these mechanisms by exposing to osmotic stress using varying polyethylene glycol (PEG) concentrations (1%, 5%, 10%) in a controlled laboratory setting. Growth analysis revealed significant inhibition and phenotypic changes with increasing PEG levels. Transcriptomic analysis, including differentially expressed gene identification, GO enrichment analysis, and hierarchical cluster analysis of genes in roots and shoots, identified key pathways associated with drought adaptation, such as ABA-activated signaling, cell wall biogenesis, photosynthesis, and secondary metabolite biosynthesis. Notably, some genes involved in these pathways exhibited tissue-specific expression patterns and showed PEG concentration-dependent regulation. Key findings include the dose-dependent (R > 0.8) upregulation of a proline-rich protein (Asp01G030840) and a BURP domain-containing protein (Asp02G039780), as well as critical genes involved in cell wall biogenesis (encoding Pectinesterase inhibitor domain-containing and Fasciclin-like arabinogalactan protein), and secondary metabolite biosynthesis (encoding enzymes for terpenoid and flavonoid biosynthesis). The regulation of these genes is likely influenced by phytohormones such as ABA and other stress-related hormones, along with significant transcription factors like ABI4, TALE, MYB61, GRAS, and ERF. These insights lay the groundwork for further research into the functional roles of these genes, their regulatory networks, and their potential applications in enhancing drought resistance in desert plants and agricultural crops.
一种以其令人印象深刻的耐旱性而闻名的沙漠灌木，在干旱条件下表现出显著的恢复力。然而，驱动其抗旱性的潜在机制在很大程度上仍未被探索。本研究旨在通过在受控实验室环境中使用不同浓度的聚乙二醇（PEG）（1%、5%、10%）对其施加渗透胁迫来研究这些机制。生长分析表明，随着PEG水平的增加，生长受到显著抑制且出现表型变化。转录组分析，包括差异表达基因鉴定、基因本体（GO）富集分析以及根和茎中基因的层次聚类分析，确定了与干旱适应相关的关键途径，如脱落酸（ABA）激活的信号传导、细胞壁生物合成、光合作用和次生代谢物生物合成。值得注意的是，参与这些途径的一些基因表现出组织特异性表达模式，并呈现出PEG浓度依赖性调控。主要发现包括富含脯氨酸的蛋白质（Asp01G030840）和含BURP结构域的蛋白质（Asp02G039780）的剂量依赖性（R>0.8）上调，以及参与细胞壁生物合成（编码含果胶酯酶抑制剂结构域和类成束蛋白阿拉伯半乳聚糖蛋白）和次生代谢物生物合成（编码萜类和黄酮类生物合成酶）的关键基因。这些基因的调控可能受ABA等植物激素和其他与胁迫相关的激素以及重要转录因子如ABI4、TALE、MYB61、GRAS和ERF的影响。这些见解为进一步研究这些基因的功能作用、它们的调控网络以及它们在增强沙漠植物和农作物抗旱性方面的潜在应用奠定了基础。
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