Rasheed Haroon, Deng Bowen, Ahmad Daraz, Bao Jinsong
Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
Int J Mol Sci. 2024 Nov 28;25(23):12795. doi: 10.3390/ijms252312795.
Genetic diversity of nutritional quality traits is crucial for potato breeding efforts to develop better varieties for the diverse market demands. In this study, the genetic diversity of 104 potato genotypes was estimated based on nutritional quality traits such as color parameters, total phenolic content, total flavonoid content, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and 2,2-azino-bis-(3-ethylbezothiazoline-6-sulphonic acid) radical scavenging potential across two environments. The results indicated that environment II, Hangzhou 2020, exhibited higher bioactive compounds and antioxidant properties than environment I, Hangzhou 2019. The colored potato accessions exhibited higher levels of total phenolic content, total flavonoid content, DPPH, and ABTS activities than the white potato accessions, indicating the superiority of the colored to white potato accessions. The genome sequencing identified 1,101,368 high-quality single-nucleotide polymorphisms (SNPs), and 141,656 insertion/deletions (Indels). A population structure analysis revealed that genotypes can be divided into two subpopulations. Genome-wide association studies (GWAS) identified 128 significant SNPs associated with potato's color, total phenolic content, total flavonoid content, and antioxidant properties. Thus, the study provides new opportunities for strategic breeding and marker-assisted selection of ideal varieties and favorable alleles to enhance bioactive compounds and health-beneficial properties.
营养品质性状的遗传多样性对于马铃薯育种工作至关重要，有助于培育出满足多样化市场需求的优良品种。在本研究中，基于颜色参数、总酚含量、总黄酮含量、2,2-二苯基-1-苦基肼（DPPH）以及2,2-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除能力等营养品质性状，对104个马铃薯基因型在两种环境下的遗传多样性进行了评估。结果表明，环境II（杭州，2020年）比环境I（杭州，2019年）表现出更高的生物活性化合物含量和抗氧化性能。有色马铃薯种质的总酚含量、总黄酮含量、DPPH和ABTS活性水平均高于白色马铃薯种质，表明有色马铃薯种质优于白色马铃薯种质。基因组测序鉴定出1,101,368个高质量单核苷酸多态性（SNP）和141,656个插入/缺失（Indel）。群体结构分析表明，基因型可分为两个亚群。全基因组关联研究（GWAS）鉴定出128个与马铃薯颜色、总酚含量、总黄酮含量和抗氧化性能相关的显著SNP。因此，本研究为战略育种以及标记辅助选择理想品种和有利等位基因以提高生物活性化合物含量和有益健康特性提供了新机遇。
