Ma Huimin, Zhang Hongcheng, Zheng Congcong, Liu Zonghui, Wang Jing, Tian Ping, Wu Zhihai, Zhang Hualiang
Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.
ISPA, INRAE, F-33140 Villenave d'Ornon, France.
Int J Mol Sci. 2024 Nov 28;25(23):12819. doi: 10.3390/ijms252312819.
Changes in root traits and rhizosphere microbiome are important ways to optimize plant phosphorus (P) efficiency and promote multifunctionality in intercropping. However, whether and how synthetic microbial communities isolated from polyculture systems can facilitate plant growth and P uptake are still largely unknown. A field experiment was first carried out to assess the rice yield and P uptake in the rice/soybean intercropping systems, and a synthetic microbial community (SynCom) isolated from intercropped rice was then constructed to elucidate the potential mechanisms of growth-promoting effects on rice growth and P uptake in a series of pot experiments. Our results showed that the yield and P uptake of intercropped rice were lower than those of rice grown in monoculture. However, bacterial networks in the rice rhizosphere were more stable in polyculture, exhibiting more hub nodes and greater modularity compared to the rice monoculture. A bacterial synthetic community (SynCom) composed of four bacterial strains (, , , sp.) significantly enhanced the biomass and P uptake of potted rice plants. These growth-promoting effects are underpinned by multiple pathways, including the direct activation of soil available P, increased root surface area and root tip number, reduced root diameter, and promotion of root-to-shoot P translocation through up-regulation of Pi transporter genes (, , , ). This study highlights the potential of harnessing synthetic microbial communities to enhance nutrient acquisition and improve crop production.
根系性状和根际微生物群落的变化是优化植物磷（P）效率和促进间作多功能性的重要途径。然而，从混作系统中分离出的合成微生物群落是否以及如何促进植物生长和磷吸收，目前仍 largely unknown。首先进行了一项田间试验，以评估水稻/大豆间作系统中的水稻产量和磷吸收，然后构建了一个从间作水稻中分离出的合成微生物群落（SynCom），以阐明在一系列盆栽试验中对水稻生长和磷吸收的促生长作用的潜在机制。我们的结果表明，间作水稻的产量和磷吸收低于单作水稻。然而，与水稻单作相比，混作中水稻根际的细菌网络更稳定，表现出更多的枢纽节点和更高的模块性。由四种细菌菌株（，，，sp.）组成的细菌合成群落（SynCom）显著提高了盆栽水稻植株的生物量和磷吸收。这些促生长作用由多种途径支撑，包括直接激活土壤有效磷、增加根表面积和根尖数量、减小根直径以及通过上调磷转运蛋白基因（，，，）促进根到地上部的磷转运。本研究强调了利用合成微生物群落提高养分获取和改善作物产量的潜力。
