Graduate Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Microbial Diversity and Utilization Research Team, Thailand Bioresource Research Center, National Center for Genetic Engineering and Biotechnology, Pathum Thani, 12120, Thailand.
Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005746.
A Gram-stain-negative, aerobic, rod-shaped, non-spore-forming and yellow-pigment-producing bacterium, designated as Sx8-5, was isolated from stem tissue of Carey in Kanchanaburi Province Thailand. The strain exhibited tricalcium phosphate solubilizing activity. Its taxonomic position was investigated using a polyphasic approach. Sx8-5 grew at 25-37 °C (optimum 30 °C), pH 6-9 (optimum 7) and with 0 and 1% NaCl (optimum 0 %). According to the 16S rRNA gene phylogeny, Sx8-5 represents a member of genus and shared the highest sequence similarities to LL02 of 99.4 % and shared sequence similarities with other species of the genus of less than 99.4 %. The whole-genome size was 5.7 Mb, comprised of one contig, with a DNA G+C content of 66 %. The average nucleotide identity using BLASTn (ANIb) or MUMMER (ANIm) values for whole genome comparisons between Sx8-5 and LL02 and six closely related type strains were 72.33-82.14 % and 83.82-87.38 %, respectively, and the digital DNA-DNA hybridization (dDDH) values ranged from 21.0 to 28.6% when compared with the type strains of the members of the genus . Major fatty acids were summed feature 8 (C 7 and/or C 6), C and summed feature 3 (C 7 and/or C 6), respectively. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, unidentified phospholipids and unidentified polar lipids. The major isoprenoid quinone was Q-10. According to results obtained using a polyphasic approach, Sx8-5 represents a novel species of the genus , the name sp. nov. is proposed. The type strain is Sx8-5 (=JCM 35076 =TBRC 15600).
一株革兰氏阴性、需氧、杆状、不产芽孢、黄色素产生菌，命名为 Sx8-5，从泰国堪察纳布里府 Carey 的茎组织中分离得到。该菌株具有磷酸三钙溶解活性。采用多相分类法研究其分类地位。Sx8-5 在 25-37°C（最佳 30°C）、pH 6-9（最佳 7）和 0-1%NaCl（最佳 0%）下生长。根据 16S rRNA 基因系统发育，Sx8-5 代表 属的一个成员，与 99.4%的 LL02 具有最高的序列相似性，与属内其他种的序列相似性低于 99.4%。全基因组大小为 5.7 Mb，由一个连续体组成，DNA G+C 含量为 66%。Sx8-5 与 LL02 及 6 株近缘模式株的全基因组比较的平均核苷酸同一性（ANIb）或 MUMMER（ANIm）值分别为 72.33-82.14%和 83.82-87.38%，数字 DNA-DNA 杂交（dDDH）值与属内成员的模式株相比，范围为 21.0-28.6%。主要脂肪酸为(summed feature 8，C7 和/或 C6)、C 和(summed feature 3，C7 和/或 C6)。极性脂类为二磷脂酰甘油、磷脂酰乙醇胺、磷脂单甲基乙醇胺、磷脂酰甘油、未鉴定的磷脂和未鉴定的极性脂类。主要异戊二烯醌为 Q-10。根据多相分类法的结果，Sx8-5 代表了 属的一个新种，提议命名为 sp. nov.。模式菌株为 Sx8-5(=JCM 35076=TBRC 15600)。
