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CePP2C19 通过调节藨草中 ABA 的敏感性赋予其耐旱性。

CePP2C19 confers tolerance to drought by regulating the ABA sensitivity in Cyperus esculentus.

机构信息

College of Life Sciences, Jilin Agricultural University, Changchun, 130118, China.

Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.

出版信息

BMC Plant Biol. 2023 Oct 28;23(1):524. doi: 10.1186/s12870-023-04522-2.

DOI:10.1186/s12870-023-04522-2
PMID:37898801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10612301/
Abstract

BACKGROUND

Tiger nut (Cyperus esculentus) is widely known as an additional source of food, oil and feed worldwide. The agricultural production of tiger nut has been greatly hindered by drought stress, reducing both yield and quality. Protein phosphatase 2 C (PP2Cs) plays an important role in plant responses to drought stress however, the molecular mechanism of PP2Cs in tiger nuts still unclear.

RESULTS

In this study, we identified a putative group A PP2C-encoding gene (CePP2C19) from tiger nut using transcriptome analysis, which is highly induced by drought stress. The transient expression assay suggested that CePP2C19 was localized to nucleus. Furthermore, the interaction between CePP2C19 and CePYR1, a coreceptor for ABA signaling, was first detected using a yeast two-hybrid assay and then verified using a bimolecular fluorescence complementation (BiFC) analysis. In addition, the transgenic Arabidopsis lines overexpressing CePP2C19 exhibited extreme tolerance to ABA and mannitol stresses during seed germination and root growth. At the mature stage, overexpression of CePP2C19 resulted in a higher tolerance to drought stress in transgenic Arabidopsis, as confirmed by a visible phenotype and several physiological parameters. Noticeably, the silencing of CePP2C19 by virus-induced gene silencing (VIGS) showed obvious reduction in drought tolerance in tiger nut plants.

CONCLUSIONS

The CePP2C19 emerges as a pivotal gene involved in the ABA signaling pathway, which likely reduce ABA sensitivity and thus enhances drought tolerance in Cyperus esculentus.

摘要

背景

块根黑麦草（Cyperus esculentus）作为一种额外的食物、油和饲料来源，在全球范围内广为人知。块根黑麦草的农业生产受到干旱胁迫的极大阻碍，导致产量和质量下降。蛋白磷酸酶 2C（PP2Cs）在植物对干旱胁迫的反应中起着重要作用，然而，PP2Cs 在块根黑麦草中的分子机制尚不清楚。

结果

本研究通过转录组分析，从块根黑麦草中鉴定出一个假定的 A 组 PP2C 编码基因（CePP2C19），该基因受干旱胁迫高度诱导。瞬时表达试验表明 CePP2C19 定位于细胞核。此外，首次通过酵母双杂交试验检测到 CePP2C19 与 ABA 信号的核心受体 CePYR1 之间的相互作用，然后通过双分子荧光互补（BiFC）分析验证了这种相互作用。此外，过表达 CePP2C19 的转基因拟南芥在种子萌发和根生长过程中对 ABA 和甘露醇胁迫表现出极端的耐受性。在成熟阶段，过表达 CePP2C19 导致转基因拟南芥对干旱胁迫的耐受性更高，这从可见表型和几个生理参数得到证实。值得注意的是，通过病毒诱导的基因沉默（VIGS）沉默 CePP2C19 导致块根黑麦草的耐旱性明显降低。

结论

CePP2C19 是参与 ABA 信号通路的关键基因，可能降低 ABA 敏感性，从而增强块根黑麦草的耐旱性。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5da/10612301/7fe316d4ec90/12870_2023_4522_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5da/10612301/b2b3abb4aeb2/12870_2023_4522_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e5da/10612301/73e03471211b/12870_2023_4522_Fig7_HTML.jpg
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