城口山地鸡生长性状的遗传多样性分析及候选基因鉴定

Genetic Diversity Analysis and Identification of Candidate Genes for Growth Traits in Chengkou Mountain Chicken.

作者信息

Liu Lingbin, Wang Yi, Huang Yu, Wang Zhen, Wang Qigui, Wang Haiwei

机构信息

Chongqing Academy of Animal Sciences, Rongchang, Chongqing 402460, China.

College of Animal Science and Technology, Southwest University, Beibei, Chongqing 400715, China.

出版信息

Int J Mol Sci. 2024 Dec 2;25(23):12939. doi: 10.3390/ijms252312939.

DOI:10.3390/ijms252312939

PMID:39684650

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11641596/

Abstract

Growth traits constitute critical factors in the breeding program of broiler chickens. The Chengkou mountain chicken A-lineage (CMC-A) represents a breed specifically bred for meat production. To further explore the growth performance of the CMC-A population, this study conducted whole-genome sequencing on 464 CMC-A roosters to systematically evaluate their genetic diversity. Additionally, runs of homozygosity (ROH) islands and genome-wide association studies (GWASs) were employed to identify the loci and functional genes influencing the growth traits in Chengkou mountain chickens. The results revealed a high level of genetic diversity and low levels of inbreeding in Chengkou mountain chickens. Several genes associated with stress resistance, muscle growth, and fat deposition were pinpointed through ROH island identification. Moreover, 52 SNP loci were detected, along with 71 candidate genes. These findings enhance our understanding of the genetic architecture underlying the growth traits in Chengkou mountain chickens and provide a theoretical foundation for subsequent breeding endeavors.

摘要

生长性状是肉鸡育种计划中的关键因素。城口山地鸡A系（CMC-A）是专门为肉类生产培育的品种。为了进一步探究CMC-A群体的生长性能，本研究对464只CMC-A公鸡进行了全基因组测序，以系统评估其遗传多样性。此外，利用纯合子片段（ROH）岛和全基因组关联研究（GWAS）来鉴定影响城口山地鸡生长性状的基因座和功能基因。结果显示，城口山地鸡具有较高的遗传多样性和较低的近亲繁殖水平。通过ROH岛鉴定确定了几个与抗应激、肌肉生长和脂肪沉积相关的基因。此外，检测到52个单核苷酸多态性（SNP）位点以及71个候选基因。这些发现加深了我们对城口山地鸡生长性状遗传结构的理解，并为后续育种工作提供了理论基础。